气候变化领域集成服务门户(CCPortal): Continuous measurements of volatile gases as detection of algae crop health

CCPortal

DOI	10.1073/pnas.2106882118
	Continuous measurements of volatile gases as detection of algae crop health
	Sauer J.S.; Simkovsky R.; Moore A.N.; Camarda L.; Sherman S.L.; Prather K.A.; Pomeroy R.S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:40
英文摘要	Algae cultivation in open raceway ponds is considered the most economical method for photosynthetically producing biomass for biofuels, chemical feedstocks, and other high-value products. One of the primary challenges for open ponds is diminished biomass yields due to attack by grazers, competitors, and infectious organisms. Higher-frequency observations are needed for detection of grazer infections, which can rapidly reduce biomass levels. In this study, real-time measurements were performed using chemical ionization mass spectrometry (CIMS) to monitor the impact of grazer infections on cyanobacterial cultures. Numerous volatile gases were produced during healthy growth periods from freshwater Synechococcus elongatus Pasteur Culture Collection (PCC) 7942, with 6-methyl-5-hepten-2-one serving as a unique metabolic indicator of exponential growth. Following the introduction of a Tetrahymena ciliate grazer, the concentrations of multiple volatile species were observed to change after a latent period as short as 18 h. Nitrogenous gases, including ammonia and pyrroline, were found to be reliable indicators of grazing. Detection of grazing by CIMS showed indicators of infections much sooner than traditional methods, microscopy, and continuous fluorescence, which did not detect changes until 37 to 76 h after CIMS detection. CIMS analysis of gases produced by PCC 7942 further shows a complex temporal array of biomass-dependent volatile gas production, which demonstrates the potential for using volatile gas analysis as a diagnostic for grazer infections. Overall, these results show promise for the use of continuous volatile metabolite monitoring for the detection of grazing in algal monocultures, potentially reducing current grazing-induced biomass losses, which could save hundreds of millions of dollars. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Algal grazers; Chemical ionization; Crop protection; Volatile organic compounds
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238351
作者单位	Department of Chemistry and Biochemistry, University of California San Diego, San diego, CA 92093, United States; Department of Biological Sciences, University of California San Diego, San diego, CA 92093, United States; Scripps Institution of Oceanography, University of California San Diego, San diego, CA 92093, United States
推荐引用方式 GB/T 7714	Sauer J.S.,Simkovsky R.,Moore A.N.,et al. Continuous measurements of volatile gases as detection of algae crop health[J],2021,118(40).
APA	Sauer J.S..,Simkovsky R..,Moore A.N..,Camarda L..,Sherman S.L..,...&Pomeroy R.S..(2021).Continuous measurements of volatile gases as detection of algae crop health.Proceedings of the National Academy of Sciences of the United States of America,118(40).
MLA	Sauer J.S.,et al."Continuous measurements of volatile gases as detection of algae crop health".Proceedings of the National Academy of Sciences of the United States of America 118.40(2021).