气候变化领域集成服务门户(CCPortal): Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits

CCPortal

DOI	10.1073/pnas.2101178118
	Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits
	Kieft B.; Li Z.; Bryson S.; Hettich R.L.; Pan C.; Mayali X.; Mueller R.S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:41
英文摘要	Blooms of marine phytoplankton fix complex pools of dissolved organic matter (DOM) that are thought to be partitioned among hundreds of heterotrophic microbes at the base of the food web. While the relationship between microbial consumers and phytoplankton DOM is a key component of marine carbon cycling, microbial loop metabolism is largely understood from model organisms and substrates. Here, we took an untargeted approach to measure and analyze partitioning of four distinct phytoplankton-derived DOM pools among heterotrophic populations in a natural microbial community using a combination of ecogenomics, stable isotope probing (SIP), and proteomics. Each 13C-labeled exudate or lysate from a diatom or a picocyanobacterium was preferentially assimilated by different heterotrophic taxa with specialized metabolic and physiological adaptations. Bacteroidetes populations, with their unique high-molecular-weight transporters, were superior competitors for DOM derived from diatom cell lysis, rapidly increasing growth rates and ribosomal protein expression to produce new relatively high C:N biomass. Proteobacteria responses varied, with relatively low levels of assimilation by Gammaproteobacteria populations, while copiotrophic Alphaproteobacteria such as the Roseobacter clade, with their diverse array of ABC- and TRAP-type transporters to scavenge monomers and nitrogen-rich metabolites, accounted for nearly all cyanobacteria exudate assimilation and produced new relatively low C:N biomass. Carbon assimilation rates calculated from SIP data show that exudate and lysate from two common marine phytoplankton are being used by taxonomically distinct sets of heterotrophic populations with unique metabolic adaptations, providing a deeper mechanistic understanding of consumer succession and carbon use during marine bloom events. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dissolved organic matter; Microbial loop; Phytoplankton bloom; Proteomics SIP; Resource partitioning
语种	英语
scopus关键词	ABC transporter; carbon 13; carrier protein; dissolved organic matter; TRAP protein; unclassified drug; algal bloom; Alphaproteobacteria; Article; Bacteroidetes; biomass; carbon fixation; controlled study; cyanobacterium; diatom; Gammaproteobacteria; growth rate; heterotroph; isotope analysis; mass spectrometry; metagenomics; microbial consortium; nonhuman; phytoplankton; protein expression; Proteobacteria; proteomics; resource partitioning; Roseobacter; Synechococcus; Thalassiosira pseudonana
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238342
作者单位	Department of Microbiology, Oregon State University, Corvallis, OR 97331, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, United States; Graduate School of Genome Science and Technology, The University of Tennessee, Knoxville, TN 37996, United States; Department of Civil & Environmental Engineering, The University of Washington, Seattle, WA 98195, United States; Department of Microbiology and Plant Microbiology, University of Oklahoma, Norman, OK 73019, United States; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, United States
推荐引用方式 GB/T 7714	Kieft B.,Li Z.,Bryson S.,et al. Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits[J],2021,118(41).
APA	Kieft B..,Li Z..,Bryson S..,Hettich R.L..,Pan C..,...&Mueller R.S..(2021).Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits.Proceedings of the National Academy of Sciences of the United States of America,118(41).
MLA	Kieft B.,et al."Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits".Proceedings of the National Academy of Sciences of the United States of America 118.41(2021).