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DOI	10.1073/pnas.2113852118
	Serotonin modulates melatonin synthesis as an autocrine neurotransmitter in the pineal gland
	Lee B.H.; Hille B.; Koh D.-S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:43
英文摘要	The pineal gland secretes melatonin principally at night. Regulated by norepinephrine released from sympathetic nerve terminals, adrenergic receptors on pinealocytes activate aralkylamine N-acetyltransferase that converts 5-hydroxytryptamine (5-HT, serotonin) to N-acetylserotonin, the precursor of melatonin. Previous studies from our group and others reveal significant constitutive secretion of 5-HT from pinealocytes. Here, using mass spectrometry, we demonstrated that the 5-HT is secreted primarily via a decynium-22–sensitive equilibrative plasma membrane monoamine transporter instead of by typical exocytotic quantal secretion. Activation of the endogenous 5-HT receptors on pinealocytes evoked an intracellular Ca2+ rise that was blocked by RS-102221, an antagonist of 5-HT2C receptors. Applied 5-HT did not evoke melatonin secretion by itself, but it did potentiate melatonin secretion evoked by submaximal norepinephrine. In addition, RS-102221 reduced the norepinephrine-induced melatonin secretion in strips of pineal gland, even when no exogenous 5-HT was added, suggesting that the 5-HT that is constitutively released from pinealocytes accumulates enough in the tissue to act as an autocrine feedback signal sensitizing melatonin release. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Melatonin; N-acetylserotonin; Pineal gland; Plasma membrane monoamine transporter; Serotonin receptor
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238330
作者单位	Department of Physiology and Biophysics, School of Medicine, University of Washington, Seattle, WA 98195, United States
推荐引用方式 GB/T 7714	Lee B.H.,Hille B.,Koh D.-S.. Serotonin modulates melatonin synthesis as an autocrine neurotransmitter in the pineal gland[J],2021,118(43).
APA	Lee B.H.,Hille B.,&Koh D.-S..(2021).Serotonin modulates melatonin synthesis as an autocrine neurotransmitter in the pineal gland.Proceedings of the National Academy of Sciences of the United States of America,118(43).
MLA	Lee B.H.,et al."Serotonin modulates melatonin synthesis as an autocrine neurotransmitter in the pineal gland".Proceedings of the National Academy of Sciences of the United States of America 118.43(2021).