气候变化领域集成服务门户(CCPortal): Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils

CCPortal

DOI	10.1073/pnas.2025322118
	Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils
	Ortiz M.; Leung P.M.; Shelley G.; Jirapanjawat T.; Nauer P.A.; Van Goethem M.W.; Bay S.K.; Islam Z.F.; Jordaan K.; Vikram S.; Chown S.L.; Hogg I.D.; Makhalanyane T.P.; Grinter R.; Cowan D.A.; Greening C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:45
英文摘要	Numerous diverse microorganisms reside in the cold desert soils of continental Antarctica, though we lack a holistic understanding of the metabolic processes that sustain them. Here, we profile the composition, capabilities, and activities of the microbial communities in 16 physicochemically diverse mountainous and glacial soils. We assembled 451 metagenome-assembled genomes from 18 microbial phyla and inferred through Bayesian divergence analysis that the dominant lineages present are likely native to Antarctica. In support of earlier findings, metagenomic analysis revealed that the most abundant and prevalent microorganisms are metabolically versatile aerobes that use atmospheric hydrogen to support aerobic respiration and sometimes carbon fixation. Surprisingly, however, hydrogen oxidation in this region was catalyzed primarily by a phylogenetically and structurally distinct enzyme, the group 1l [NiFe]-hydrogenase, encoded by nine bacterial phyla. Through gas chromatography, we provide evidence that both Antarctic soil communities and an axenic Bacteroidota isolate (Hymenobacter roseosalivarius) oxidize atmospheric hydrogen using this enzyme. Based on ex situ rates at environmentally representative temperatures, hydrogen oxidation is theoretically sufficient for soil communities to meet energy requirements and, through metabolic water production, sustain hydration. Diverse carbon monoxide oxidizers and abundant methanotrophs were also active in the soils. We also recovered genomes of microorganisms capable of oxidizing edaphic inorganic nitrogen, sulfur, and iron compounds and harvesting solar energy via microbial rhodopsins and conventional photosystems. Obligately symbiotic bacteria, including Patescibacteria, Chlamydiae, and predatory Bdellovibrionota, were also present. We conclude that microbial diversity in Antarctic soils reflects the coexistence of metabolically flexible mixotrophs with metabolically constrained specialists. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Actinobacteria; Antarctica; Hydrogen; Metabolic water; Trace gas
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238318
作者单位	Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, 0002, South Africa; Department of Microbiology, Monash Biomedicine Discovery Institute, Clayton, VIC 3800, Australia; School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia; School of Chemistry, Monash University, Clayton, VIC 3800, Australia; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, 8331150, Chile; Securing Antarctica's Environmental Future, School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia; School of Science, University of Waikato, Hamilton, 3240, New Zealand; Polar Knowledge Canada, Canadian High Arctic Research Station, Cambridge, Bay NU X0B 0C0, Canada
推荐引用方式 GB/T 7714	Ortiz M.,Leung P.M.,Shelley G.,et al. Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils[J],2021,118(45).
APA	Ortiz M..,Leung P.M..,Shelley G..,Jirapanjawat T..,Nauer P.A..,...&Greening C..(2021).Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils.Proceedings of the National Academy of Sciences of the United States of America,118(45).
MLA	Ortiz M.,et al."Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils".Proceedings of the National Academy of Sciences of the United States of America 118.45(2021).