气候变化领域集成服务门户(CCPortal): Comparison of fecal indicators with pathogenic bacteria and rotavirus in rural Bangladesh groundwater

CCPortal

DOI	https://doi.org/10.1594/PANGAEA.774444
	Comparison of fecal indicators with pathogenic bacteria and rotavirus in rural Bangladesh groundwater
	Ferguson; Andrew; Layton; Alice; Mailloux; Brian; Culligan; Patricia; Williams; Daniel; Smartt; Abby; Sayler; Gary; Feighery; John; McKay; Larry; Knappett; Peter; Alexandrova; Ekaterina; Arbit; Talia; Emch; Michael; Escamilla; Veronica; Ahmed; Kazi Matin; Alam; Md Jahangir; Streatfield; P Kim; Yunus; Mohammad; van Geen; Alexander
发布日期	2012-01-20
数据集类型	dataset
英文简介	Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria. Five indicator microorganisms (E. coli, total coliform, F+RNA coliphage, Bacteroides and human-associated Bacteroides (HuBacteroides)) and various environmental parameters were compared to the direct detection of waterborne pathogens by quantitative PCR in groundwater pumped from 50 tubewells. Rotavirus was detected in groundwater filtrate from the largest proportion of tubewells (40%), followed by Shigella (10%), Vibrio (10%), and pathogenic E. coli (8%). Spearman rank correlations and sensitivity-specificity calculations indicate that some, but not all, combinations of indicators and environmental parameters can predict the presence of pathogens. Culture-dependent fecal indicator bacteria measured on a single date did not predict bacterial pathogens, but annually averaged monthly measurements of culturable E. coli did improve prediction for total bacterial pathogens. F+RNA coliphage were neither correlated nor sufficiently sensitive towards rotavirus, but were predictive of bacterial pathogens. A qPCR-based E. coli assay was the best indicator for the bacterial pathogens, rotavirus and all pathogens combined. Since groundwater cannot be excluded as a significant source of diarrheal disease in Bangladesh and neighboring countries with similar characteristics, the need to develop more effective methods for screening tubewells with respect to microbial contamination is necessary.
空间范围	Median Latitude: 23.369780 * Median Longitude: 90.646520 * South-bound Latitude: 23.364000 * West-bound Longitude: 90.644000 * North-bound Latitude: 23.373000 * East-bound Longitude: 90.651000
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/217807
推荐引用方式 GB/T 7714	Ferguson,Andrew,Layton,et al. Comparison of fecal indicators with pathogenic bacteria and rotavirus in rural Bangladesh groundwater.2012-01-20.https://doi.org/10.1594/PANGAEA.774444.