气候变化领域集成服务门户(CCPortal): Chemical characterization and quantification of the brown algal storage compound laminarin

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DOI	https://doi.org/10.1594/PANGAEA.843456
	Chemical characterization and quantification of the brown algal storage compound laminarin
	Graiff; Angelika; Ruth; Wolfgang; Kragl; Udo; Karsten; Ulf
发布日期	2015-03-03
数据集类型	dataset
英文简介	In search of a meaningful stress indicator for Fucus vesiculosus we found that the often used quantitative determination procedures for the polysaccharide laminarin (beta-1,3-glucan) result in different kind of problems, uncertainties and limitations. This chemical long-term storage form of carbon enables perennial brown algae in seasonally fluctuating ecosystems to uncouple growth from photosynthesis. Because of this high ecological relevance a reliable and precise method for determination and quantification of laminarin is needed. Therefore, a simple, cold water extraction method coupled to a new quantitative liquid chromatography-mass spectrometrical method (LC-MS) was developed. Laminarin was determined in nine out of twelve brown algal species, and its expected typical molar mass distribution of 2000-7000 Da was confirmed. Furthermore, laminarin consisted of a complex mixture of different chemical forms, since fifteen chemical laminarin species with distinct molecular weights were measured in nine species of brown algae. Laminarin concentrations in the algal tissues ranged from 0.03 to 0.86% dry weight (DW). The direct chemical characterization and quantification of laminarin by LC-MS represents a powerful method to verify the biochemical and ecological importance of laminarin for brown algae.
空间范围	Latitude: 54.156944 * Longitude: -9.116110
时间范围	2013-11-18T00:00:00 - 2013-11-18T00:00:00
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/217173
推荐引用方式 GB/T 7714	Graiff,Angelika,Ruth,et al. Chemical characterization and quantification of the brown algal storage compound laminarin.2015-03-03.https://doi.org/10.1594/PANGAEA.843456.