气候变化领域集成服务门户(CCPortal): Composition of bacterioplankton in the North Atlantic subtropical gyre

CCPortal

DOI	https://doi.pangaea.de/10.1594/PANGAEA.860819
	Composition of bacterioplankton in the North Atlantic subtropical gyre
	Gomez-Pereira; Paola R; Hartmann; Manuela; Grob; C; Tarran; Glen A; Martin; Adrian Peter; Fuchs; Bernhard M; Scanlan; David J; Zubkov; Mikhail V
发布日期	2016-05-27
数据集类型	dataset
英文简介	Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoheterotrophic. We assessed the photoheterotrophy of the key microbial taxa in the North Atlantic oligotrophic gyre and adjacent regions. The experimental work was performed on board the Royal Research Ship James Cook (cruise no. JC53, October-November 2010) as part of the Atlantic Meridional Transect programme, and on board the Royal Research Ship Discovery (cruise no. D369, August-September 2011). At each station, samples were collected from 20m depth with a sampling rosette of 20-l Niskin bottles mounted on aconductivity-temperature-depth profiler. Samples were collected in 1 l thermos flasks (washed with10% v/v HCl) in the dark and processed immediately. Depth of 20m was chosen because it represents the mixed layer and it was the shallowest depth unaffected by the ship's movement, including thrusting, that could artificially affect microbial metabolism in nutrient-depleted stratified surfacewaters. Molecular identification of flow-sorted cells CARD-FISH was performed on flow-sorted cells to identify the groups for which uptake rates were measured. High nucleic acid-containing bacteria, based on SYBR Green DNA staining, that had virtually undetectable chlorophyll autofluorescence, were phylogenetically affiliated with Prochlorococcus,in agreement with our previously reported results (Zubkov et al., 2007; doi:10.1111/j.1462-2920.2007.01324.x).
空间范围	Median Latitude: 21.374000 * Median Longitude: 33.906000 * South-bound Latitude: 4.800000 * West-bound Longitude: 28.160000 * North-bound Latitude: 34.210000 * East-bound Longitude: 41.110000
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/216837
推荐引用方式 GB/T 7714	Gomez-Pereira,Paola R,Hartmann,et al. Composition of bacterioplankton in the North Atlantic subtropical gyre.2016-05-27.https://doi.pangaea.de/10.1594/PANGAEA.860819.