气候变化领域集成服务门户(CCPortal): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

CCPortal

DOI	https://doi.org/10.1594/PANGAEA.867318
	Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
	Dineshram; R; Chandramouli; K; Ko; W K Ginger; Zhang; Huoming; Qian; Pei Yuan; Ravasi; Timothy; Thiyagarajan; Vengatesen
发布日期	2016-10-28
数据集类型	dataset
英文关键词	Animalia ; Coast and continental shelf ; Containers and aquaria (20- 1000 L or &lt ; 1 m**2) ; Crassostrea gigas ; Gene expression (incl. proteomics) ; Laboratory experiment ; Mollusca ; Mortality/Survival ; North Pacific ; Pelagos ; Salinity ; Single species ; Temperate ; Temperature ; Zooplankton
英文简介	The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.
空间范围	Latitude: 36.066670 * Longitude: 120.366670
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/216741
推荐引用方式 GB/T 7714	Dineshram,R,Chandramouli,et al. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors.2016-10-28.https://doi.org/10.1594/PANGAEA.867318.