气候变化领域集成服务门户(CCPortal): Development of bacterial and archaeal communities in erupted subsurface muds at the Håkon Mosby mud volcano

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DOI	https://doi.org/10.1594/PANGAEA.861266
	Development of bacterial and archaeal communities in erupted subsurface muds at the Håkon Mosby mud volcano
	Ruff; S Emil; Felden; Janine; Marcon; Yann; Ramette; Alban; Boetius; Antje
发布日期	2016-06-07
数据集类型	dataset
英文简介	Emission of the greenhouse gas methane from the seabed is globally controlled by marine aerobic and anaerobic methanotrophs gaining energy via methane oxidation. However, the processes involved in the assembly and dynamics of methanotrophic populations in complex natural microbial communities remain unclear. Here we investigated the development of a methanotrophic microbiome following subsurface mud eruptions at Håkon Mosby mud volcano (1250 m water depth). We analyzed surface and subsurface sediment samples across HMMV mud flows from most recently discharged subsurface muds towards old consolidated muds as well as one reference site located approximately 0.5 km outside of the HMMV. Surface sediment samples (0-20 cm) were recovered either by TV-guided Multicorer or by push cores. Subsurface sediments of all zones (>2 m below sea floor) were obtained in by gravity corer. After recovery, sediments were immediately subsampled in a refrigerated container (0°C) and further processed for biogeochemical analyses or preserved at -20°C for later DNA analyses. Our study show that freshly erupted muds hosted deep-subsurface communities, which were dominated by Bathyarchaeota, Atribacteria and Chloroflexi. Methanotrophy was initially limited to a thin surface layer of Methylococcales populations consuming methane aerobically. With increasing distance to the eruptive center, anaerobic methanotrophic archaea, sulfate-reducing Desulfobacterales and thiotrophic Beggiatoaceae developed, and their respective metabolic capabilities dominated the biogeochemical functions of the community. Microbial richness, evenness, and cell numbers of the entire microbial community increased up to tenfold within a few years downstream of the mud flow from the eruptive center. The increasing diversity was accompanied by an up to fourfold increase in sequence abundance of relevant metabolic genes of the anaerobic methanotrophic and thiotrophic guilds. The communities fundamentally changed in their structure and functions as reflected in the metagenome turnover with distance from the eruptive center, and this was reflected in the biogeochemical zonation across the mud volcano caldera. The observed functional succession provides a framework for the response time and recovery of complex methanotrophic communities after disturbances of the deep-sea bed.
空间范围	Median Latitude: 72.004234 * Median Longitude: 14.730608 * South-bound Latitude: 71.975000 * West-bound Longitude: 14.683333 * North-bound Latitude: 72.016667 * East-bound Longitude: 14.775000
时间范围	2003-06-28T10:02:00 - 2010-10-04T13:01:00
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/215399
推荐引用方式 GB/T 7714	Ruff,S Emil,Felden,et al. Development of bacterial and archaeal communities in erupted subsurface muds at the Håkon Mosby mud volcano.2016-06-07.https://doi.org/10.1594/PANGAEA.861266.