气候变化领域集成服务门户(CCPortal): Histological and relative gene expression data obtained from the gills of juvenile turbot (Psetta maxima) after the exposure to varying degrees of severe environmental hypercapnia for 8 weeks.

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DOI	https://doi.org/10.1594/PANGAEA.893884
	Histological and relative gene expression data obtained from the gills of juvenile turbot (Psetta maxima) after the exposure to varying degrees of severe environmental hypercapnia for 8 weeks.
	Hermann; Bernd Timo; Würtz; Sven; Vanselow; Klaus Heinrich; Schulz; Carsten; Stiller; Kevin Torben
发布日期	2018-09-11
数据集类型	dataset
英文简介	The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm.
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/215213
推荐引用方式 GB/T 7714	Hermann,Bernd Timo,Würtz,et al. Histological and relative gene expression data obtained from the gills of juvenile turbot (Psetta maxima) after the exposure to varying degrees of severe environmental hypercapnia for 8 weeks..2018-09-11.https://doi.org/10.1594/PANGAEA.893884.