气候变化领域集成服务门户(CCPortal): Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development

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DOI	10.1016/j.scib.2021.06.010
	Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development
	Zhu Y.; Yu J.; Rong Y.; Wu Y.-W.; Li Y.; Zhang L.; Pan Y.; Fan H.-Y.; Shen L.
发表日期	2021
ISSN	20959273
英文摘要	Polycomb group (PcG) proteins are crucial chromatin regulators during development. H2AK119ub1 (H2Aub) and H3K27me3 are catalyzed by Polycomb-repressive complex 1 and 2 (PRC1/2) respectively, and they largely overlap in the genome due to mutual recruitment of the two complexes. However, it is unclear whether PRC1/H2Aub and PRC2/H3K27me3 can also function independently. By developing an ultra-sensitive carrier-DNA-assisted chromatin immunoprecipitation sequencing method termed CATCH-Seq, we generated allelic H2Aub profiles in mouse gametes and early embryos. Our results revealed an unexpected genomewide decoupling of H2Aub and H3K27me3 in mouse preimplantation embryos, where H2Aub but not H3K27me3 was enriched at PcG targets while only H3K27me3 was deposited in the broad distal domains associated with DNA methylation-independent non-canonical imprinting. These observations suggest that H2Aub represses future bivalent genes during early embryogenesis without H3K27me3, but it is not required for the maintenance of non-canonical imprinting, which is mediated by maternal H3K27me3. Thus, our study reveals the distinct depositions and independent functions of H2Aub and H3K27me3 during early mammalian development. © 2021 Science China Press
关键词	H2AK119ub1 H3K27me3 Non-canonical imprinting Polycomb Preimplantation development
英文关键词	Alkylation; Gene encoding; Chromatin immunoprecipitation; Complex 1; Decouplings; H2ak119ub1; H3k27me3; Non-canonical imprinting; Polycomb; Polycomb group proteins; Preimplantation development; Ultra sensitives; Mammals
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207700
作者单位	The MOE Key Laboratory of Biosystems Homeostasis & Protection and Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, 310058, China; Department of Orthopedics Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Hangzhou Innovation Center, Zhejiang University, Hangzhou, 311215, China
推荐引用方式 GB/T 7714	Zhu Y.,Yu J.,Rong Y.,et al. Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development[J],2021.
APA	Zhu Y..,Yu J..,Rong Y..,Wu Y.-W..,Li Y..,...&Shen L..(2021).Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development.Science Bulletin.
MLA	Zhu Y.,et al."Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development".Science Bulletin (2021).