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| DOI | 10.1016/j.scib.2021.03.010 |
| A mirror-image protein-based information barcoding and storage technology | |
| Zheng J.-S.; Liang J.; Shi W.-W.; Li Y.; Hu H.-G.; Tian C.-L.; Liu L. | |
| 发表日期 | 2021 |
| ISSN | 20959273 |
| 起始页码 | 1542 |
| 结束页码 | 1549 |
| 卷号 | 66期号:15 |
| 英文摘要 | A mirror-image protein-based information barcoding and storage technology wherein D-amino acids are used to encode information into mirror-image proteins that are chemically synthesized is described. These mirror-image proteins were then fused into various materials from which information-encoded objects were produced. Subsequently, the mirror-image proteins were extracted from the objects using biotin-streptavidin resin-mediated specific enrichment and cleaved using an Ni(II)-mediated selective peptide cleavage. Protein sequencing was accomplished using liquid chromatography/tandem mass spectrometry (LC-MS/MS) and then transcoded into the recorded information. We demonstrated the use of this technology to encode Chinese words into mirror-image proteins, which were then fused onto a poly(ethylene terephthalate) (PET) film and retrieved and decoded by LC-MS/MS sequencing. Compared to information barcoding and storage technologies using natural biopolymers, the mirror-image biopolymers used in our technology may be more stable and durable. © 2021 Science China Press |
| 关键词 | Chemical protein synthesisInformation barcoding and storageMirror-image proteinsProtein sequencingProtein-of-things |
| 英文关键词 | Biomolecules; Encoding (symbols); Ethylene; Liquid chromatography; Mass spectrometry; Mirrors; Nickel compounds; Storage (materials); Biotin-streptavidin; Chemical protein synthesis; D-amino acid; Information barcoding and storage; Mirror images; Mirror-image protein; Protein sequencing; Protein-of-thing; Storage technology; Synthesised; Proteins |
| 语种 | 英语 |
| 来源期刊 | Science Bulletin
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/207691 |
| 作者单位 | Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China; Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China; Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China |
| 推荐引用方式 GB/T 7714 | Zheng J.-S.,Liang J.,Shi W.-W.,et al. A mirror-image protein-based information barcoding and storage technology[J],2021,66(15). |
| APA | Zheng J.-S..,Liang J..,Shi W.-W..,Li Y..,Hu H.-G..,...&Liu L..(2021).A mirror-image protein-based information barcoding and storage technology.Science Bulletin,66(15). |
| MLA | Zheng J.-S.,et al."A mirror-image protein-based information barcoding and storage technology".Science Bulletin 66.15(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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