气候变化领域集成服务门户(CCPortal): A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes

CCPortal

DOI	10.1016/j.scib.2020.09.004
	A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes
	Zhao X.; Li S.; Liu G.; Wang Z.; Yang Z.; Zhang Q.; Liang M.; Liu J.; Li Z.; Tong Y.; Zhu G.; Wang X.; Jiang L.; Wang W.; Tan G.-Y.; Zhang L.
发表日期	2021
ISSN	20959273
起始页码	69
结束页码	77
卷号	66 期号:1
英文摘要	Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR–Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes. © 2020 Science China Press
关键词	Alpha fetoprotein detection Aptamer Biosensing platform Cocaine detection CRISPR–Cas12a Diverse analyte
英文关键词	Tumors; Alpha fetoprotein; Biosensing platforms; Detection limits; ITS applications; Nucleic acid detection; Sensitive detection; Single strand DNA; State of the art; Nucleic acids
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207686
作者单位	State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology (ECUST), Shanghai, 200237, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; Department of Urology, Sixth People's Hospital South Campus Affiliated to Shanghai Jiao Tong University, Shanghai, 201499, China; Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, 2800, Denmark; University of Chinese Academy of Sciences, Beijing, 100049, China
推荐引用方式 GB/T 7714	Zhao X.,Li S.,Liu G.,等. A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes[J],2021,66(1).
APA	Zhao X..,Li S..,Liu G..,Wang Z..,Yang Z..,...&Zhang L..(2021).A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes.Science Bulletin,66(1).
MLA	Zhao X.,et al."A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes".Science Bulletin 66.1(2021).