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DOI	10.1016/j.scib.2021.03.003
	A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria
	Yu L.; Cao Q.; Chen W.; Yang N.; Yang C.-G.; Ji Q.; Wu M.; Bae T.; Lan L.
发表日期	2021
ISSN	20959273
英文摘要	In nature, bacteria must sense copper and tightly regulate gene expression to evade copper toxicity. Here, we identify a new copper-responsive two-component system named DsbRS in the important human pathogen Pseudomonas aeruginosa; in this system, DsbS is a sensor histidine kinase, and DsbR, its cognate response regulator, directly induces the transcription of genes involved in protein disulfide bond formation (Dsb) (i.e., the dsbDEG operon and dsbB). In the absence of copper, DsbS acts as a phosphatase toward DsbR, thus blocking the transcription of Dsb genes. In the presence of copper, the metal ion directly binds to the sensor domain of DsbS, and the Cys82 residue plays a critical role in this process. The copper-binding behavior appears to inhibit the phosphatase activity of DsbS, leading to the activation of DsbR. The copper resistance of the dsbRS knock-out mutant is restored by the ectopic expression of the dsbDEG operon, which is a DsbRS major target. Strikingly, cognates of the dsbRS-dsbDEG pair are widely distributed across eubacteria. In addition, a DsbR-binding site, which contains the consensus sequence 5′-TTA-N8-TTAA-3′, is detected in the promoter region of dsbDEG homologs in these species. These findings suggest that the regulation of Dsb genes by DsbRS represents a novel mechanism by which bacterial cells cope with copper stress. © 2021 Science China Press
关键词	Copper resistance Disulfide bond formation Gene regulation Pseudomonas aeruginosa Two-component system
英文关键词	Amino acids; Copper; Covalent bonds; Metal ions; Phosphatases; Sulfur compounds; Transcription; Copper resistance; Copper toxicity; Disulfide bond formation; Gene-regulation; Genes expression; Histidine kinase; Human pathogens; Pseudomonas aeruginosa; Response regulators; Two component systems; Bacteria
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207650
作者单位	University of Chinese Academy of Sciences, Beijing, 100049, China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Department of Biomedical Sciences, University of North DakotaGrand Forks ND 58203-9037, United States; Department of Microbiology and Immunology, Indiana University School of Medicine-NorthwestGary IN 46408, United States; School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China; NMPA Key Laboratory for Testing Technology of Pharmaceutical Microbiology, Shanghai Institute for Food and Drug Control, Shanghai, 201203, China
推荐引用方式 GB/T 7714	Yu L.,Cao Q.,Chen W.,et al. A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria[J],2021.
APA	Yu L..,Cao Q..,Chen W..,Yang N..,Yang C.-G..,...&Lan L..(2021).A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria.Science Bulletin.
MLA	Yu L.,et al."A novel copper-sensing two-component system for inducing Dsb gene expression in bacteria".Science Bulletin (2021).