气候变化领域集成服务门户(CCPortal): A cilium-independent role for intraflagellar transport 88 in regulating angiogenesis

CCPortal

DOI	10.1016/j.scib.2020.10.014
	A cilium-independent role for intraflagellar transport 88 in regulating angiogenesis
	Yang Y.; Chen M.; Li J.; Hong R.; Yang J.; Yu F.; Li T.; Yang S.; Ran J.; Guo C.; Zhao Y.; Luan Y.; Liu M.; Li D.; Xie S.; Zhou J.
发表日期	2021
ISSN	20959273
起始页码	727
结束页码	739
卷号	66 期号:7
英文摘要	Endothelial cilia are microtubule-based hair-like protrusions in the lumen of blood vessels that function as fluid mechanosensors to regulate vascular hemodynamics. However, the functions of endothelial cilia in vascular development remain controversial. In this study, depletion of several key proteins responsible for ciliogenesis allows us to identify a cilium-independent role for intraflagellar transport 88 (IFT88) in mammalian angiogenesis. Disruption of primary cilia by heat shock does not affect the angiogenic process. However, depletion of IFT88 significantly inhibits angiogenesis both in vitro and in vivo. IFT88 mediates angiogenesis by regulating the migration, polarization, proliferation, and oriented division of vascular endothelial cells. Further mechanistic studies demonstrate that IFT88 interacts with γ-tubulin and microtubule plus-end tracking proteins and promotes microtubule stability. Our findings indicate that IFT88 regulates angiogenesis through its actions in microtubule-based cellular processes, independent of its role in ciliogenesis. © 2020 Science China Press
关键词	Angiogenesis Cell division Cell migration Cilium IFT88 Microtubule stability
英文关键词	Endothelial cells; Mammals; Proteins; Angiogenic process; Cellular process; Intraflagellar transports; Mechanistic studies; Mechanosensors; Microtubule stability; Vascular development; Vascular endothelial cells; Blood vessels
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207640
作者单位	College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials of the Ministry of Education, Tianjin Key Laboratory of Protein Science, Nankai University, Tianjin, 300071, China; College of Life Sciences, Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Institute of Biomedical Sciences, Shandong Normal University, Jinan, 250014, China; Department of Translational Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China
推荐引用方式 GB/T 7714	Yang Y.,Chen M.,Li J.,et al. A cilium-independent role for intraflagellar transport 88 in regulating angiogenesis[J],2021,66(7).
APA	Yang Y..,Chen M..,Li J..,Hong R..,Yang J..,...&Zhou J..(2021).A cilium-independent role for intraflagellar transport 88 in regulating angiogenesis.Science Bulletin,66(7).
MLA	Yang Y.,et al."A cilium-independent role for intraflagellar transport 88 in regulating angiogenesis".Science Bulletin 66.7(2021).