气候变化领域集成服务门户(CCPortal): An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance

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DOI	10.1016/j.scib.2021.03.015
	An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance
	Shen S.; Peng M.; Fang H.; Wang Z.; Zhou S.; Jing X.; Zhang M.; Yang C.; Guo H.; Li Y.; Lei L.; Shi Y.; Sun Y.; Liu X.; Xu C.; Tohge T.; Yuan M.; Fernie A.R.; Ning Y.; Wang G.; Luo J.
发表日期	2021
ISSN	20959273
英文摘要	Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identification of a newly discovered hydroxycinnamoyl tyramine (HT) gene cluster in rice. This cluster contains a pyridoxamine 5′-phosphate oxidase (OsPDX3) producing the cofactor pyridoxal 5′-phosphate (PLP), a PLP-dependent tyrosine decarboxylase (OsTyDC1), and two duplicated hydroxycinnamoyl transferases (OsTHT1 and OsTHT2). These members were combined to represent an enzymological innovation gene cluster. Natural variation analysis showed that the abundance of the toxic tyramine intermediate of the gene cluster among different rice accessions is mainly determined by the coordinated transcription of OsTyDC1 and OsTHT1. Further pathogen incubation assays demonstrated that the end products of the HT gene cluster displayed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. Oryzae (Xoo) and fungal pathogen Magnaporthe oryzae (M. oryzae), and the enhanced resistance is associated with the boost of phytoalexins and the activation of defense response. The unique presence of the HT gene cluster in Oryza AA genome, together with the enrichment of transposon elements within this gene cluster region, provides an evolutionary background to accelerate cluster member combinations. Our study not only discovered a gene cluster involved in the phenylpropanoid metabolism but also addressed the key aspects of gene cluster formation. In addition, our results provide a new metabolic pool for plant defense against pathogens. © 2021 Science China Press
关键词	Gene cluster Hydroxycinnamic acid amide Magnaporthe oryzae Phytoalexin Rice Transposon element Xanthomonas oryzae pv. oryzae
英文关键词	Amines; Amino acids; Biochemistry; Metabolism; Network security; Pathogens; Transcription; Disease resistance; Gene clusters; Hydroxycinnamic acid amides; Magnaporthe oryzae; Oryza; Phytoalexin; Pyridoxal 5' phosphates; Rice; Transposon element; Xanthomonas oryza pv.; Amides
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207546
作者单位	National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China; College of Tropical Crops, Hainan University, Haikou, 570228, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Department of Plant Pathology, Ohio State University, Columbus, OH 43210, United States; VIB-UGent Center for Plant Systems Biology, Ghent University, Ghent, 9052, Belgium; Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, 14476, Germany; Centre of Plant Systems Biology and Biotechnology, Plovdiv, 4000, Bulgaria; Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan
推荐引用方式 GB/T 7714	Shen S.,Peng M.,Fang H.,et al. An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance[J],2021.
APA	Shen S..,Peng M..,Fang H..,Wang Z..,Zhou S..,...&Luo J..(2021).An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance.Science Bulletin.
MLA	Shen S.,et al."An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance".Science Bulletin (2021).