气候变化领域集成服务门户(CCPortal): m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish

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DOI	10.1016/j.scib.2021.02.003
	m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish
	Ren F.; Miao R.; Xiao R.; Mei J.
发表日期	2021
ISSN	20959273
起始页码	1119
结束页码	1128
卷号	66 期号:11
英文摘要	Bucky ball (Buc) is involved in germ plasm (GP) assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism. The present study demonstrates that an m6A reader Igf2bp3 interacts and colocalizes with Buc in the GP. Similar to the loss of Buc, the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification, which can be partially restored by the injection of igf2bp3 mRNA. Igf2bp3 binds to m6A-modified GP-organizer and GP mRNAs in an m6A-dependent manner and prevents their degradation. These findings indicate that the functions of Igf2bp3, a direct effector protein of Buc, in GP mRNA expression and GP assembly involve m6A-dependent regulation; these results emphasize a critical role of m6A modification in the process of GP assembly. © 2021 Science China Press
关键词	Bucky ball Germ cell specification Germ plasm assembly Igf2bp3 m6A reader m6A-dependent regulation
英文关键词	Gene expression; Bucky ball; Genes expression; Germ cell specification; Germ plasm assembly; Igf2bp3; M6A reader; M6A-dependent regulation; mRNA expression; Zebrafish; Zebrafish development; Specifications
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207543
作者单位	College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan, 430071, China; Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, 430071, China
推荐引用方式 GB/T 7714	Ren F.,Miao R.,Xiao R.,et al. m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish[J],2021,66(11).
APA	Ren F.,Miao R.,Xiao R.,&Mei J..(2021).m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish.Science Bulletin,66(11).
MLA	Ren F.,et al."m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish".Science Bulletin 66.11(2021).