气候变化领域集成服务门户(CCPortal): Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells

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DOI	10.1016/j.scib.2021.03.005
	Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells
	Guo W.; Wang S.; Zhang X.; Shi M.; Duan F.; Hao J.; Gu K.; Quan L.; Wu Y.; Liang Z.; Wang Y.
发表日期	2021
ISSN	20959273
起始页码	1319
结束页码	1329
卷号	66 期号:13
英文摘要	Enhanced glycolysis is a distinct feature associated with numerous stem cells and cancer cells. However, little is known about its regulatory roles in gene expression and cell fate determination. Here, we confirm that glycolytic metabolism and lactate production decrease during the differentiation of mouse embryonic stem cells (mESCs). Importantly, acidic pH due to lactate accumulation can transiently prevent the silencing of mESC self-renewal in differentiation conditions. Furthermore, acidic pH partially blocks the differentiation of human ESCs (hESCs). Mechanistically, acidic pH downregulates AGO1 protein and de-represses a subset of mRNA targets of miR-290/302 family of microRNAs which facilitate the exit of naive pluripotency state in mESCs. Interestingly, AGO1 protein is also downregulated by acidic pH in cancer cells. Altogether, this study provides insights into the potential function and underlying mechanism of acidic pH in pluripotent stem cells (PSCs). © 2021 Science China Press
关键词	Acidic pH Embryonic stem cells Glycolysis Lactate Pluripotency
英文关键词	Cytology; Diseases; Gene expression; Pathology; Proteins; RNA; Acidic pH; Cancer cells; Cell-fate; Condition; Embryonic stem cells; Genes expression; Mice embryonic stem cells; Pluripotency; Self renewal; Stem-cell; Stem cells
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207414
作者单位	Department of Medical Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China; Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing, 100871, China; Department of Pathology, Molecular Pathology Research Centre, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China
推荐引用方式 GB/T 7714	Guo W.,Wang S.,Zhang X.,et al. Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells[J],2021,66(13).
APA	Guo W..,Wang S..,Zhang X..,Shi M..,Duan F..,...&Wang Y..(2021).Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells.Science Bulletin,66(13).
MLA	Guo W.,et al."Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells".Science Bulletin 66.13(2021).