气候变化领域集成服务门户(CCPortal): Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila

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DOI	10.1016/j.scib.2021.06.019
	Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila
	Gao Y.; Liu S.; Jia Q.; Wu L.; Yuan D.; Li E.Y.; Feng Q.; Wang G.; Palli S.R.; Wang J.; Li S.
发表日期	2021
ISSN	20959273
英文摘要	Juvenile hormone (JH) and 20-hydroxyecdysone (20E) coordinately regulate development and metamorphosis in insects. Two JH intracellular receptors, methoprene-tolerant (Met) and germ-cell expressed (Gce), have been identified in the fruit fly Drosophila melanogaster. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases. Functioning through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated protein kinase C (PKC) which phosphorylated ultraspiracle (USP) at Ser35, the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP. The uspS35A mutant, in which Ser was replaced with Ala at position 35 by genome editing, showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus attenuated 20E signaling that delayed developmental timing. The uspS35A mutant also showed lower Yorkie activity that reduced body size. Altogether, JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20E action that regulates the normal fly development. This study helps better understand the complex JH signaling network. © 2021 Science China Press
关键词	20-Hydroxyecdysone action Juvenile hormone Phosphoproteomics Receptor tyrosine kinase USP Yorkie activity
英文关键词	Biochemistry; Cell signaling; Complex networks; Enzymes; Gene expression; Hormones; Phosphorylation; Signaling; 20-hydroxyecdysone action; Drosophilla; Intracellular receptors; Juvenile hormone; Methoprene; Phosphoproteomics; Protein kinases c; Receptor-tyrosine kinase; Ultraspiracle; Yorkie activity; Amino acids
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207403
作者单位	Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, 510631, China; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou, 514779, China; International Department, The Affiliated High School of South China Normal University, Guangzhou, 510631, China; Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China; Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, 40546, United States; Department of Entomology, University of Maryland, College Park, 20742, United States
推荐引用方式 GB/T 7714	Gao Y.,Liu S.,Jia Q.,et al. Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila[J],2021.
APA	Gao Y..,Liu S..,Jia Q..,Wu L..,Yuan D..,...&Li S..(2021).Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila.Science Bulletin.
MLA	Gao Y.,et al."Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila".Science Bulletin (2021).