气候变化领域集成服务门户(CCPortal): A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice

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DOI	10.1016/j.scib.2021.06.014
	A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice
	Fang H.; Shen S.; Wang D.; Zhang F.; Zhang C.; Wang Z.; Zhou Q.; Wang R.; Tao H.; He F.; Yang C.; Peng M.; Jing X.; Hao Z.; Liu X.; Luo J.; Wang G.-L.; Ning Y.
发表日期	2021
ISSN	20959273
英文摘要	Phenolamides (PAs), a diverse group of specialized metabolites, including hydroxycinnamoylputrescine (HP), hydroxycinnamoylagmatine, and hydroxycinnamoyltryptamine, are important in plant resistance to biotic stress. However, the genes involved in the biosynthesis and modulation of PAs have not been fully elucidated. This study identified an HP biosynthetic gene cluster in rice (Oryza sativa) comprising one gene (OsODC) encoding a decarboxylase and two tandem-duplicated genes (OsPHT3 and OsPHT4) encoding putrescine hydroxycinnamoyl acyltransferases coexpressed in different tissues. OsODC catalyzes the conversion of ornithine to putrescine, which is used in HP biosynthesis involving OsPHT3 and OsPHT4. OsPHT3 or OsPHT4 overexpression causes HP accumulation and cell death and putrescine hydroxycinnamoyl acyltransferases (PHT) activity-dependent resistance against the fungal pathogen Magnaporthe oryzae. OsODC overexpression plants also confer enhanced resistance to M. oryzae. Notably, the basic leucine zipper transcription factor APIP5, a negative regulator of cell death, directly binds to the OsPHT4 promoter, repressing its transcription. Moreover, APIP5 suppression induces OsPHT4 expression and HP accumulation. Comparative genomic analysis revealed that the HP biosynthetic gene cluster is conserved in monocots. These results characterized a previously unidentified monocot-specific gene cluster that is involved in HP biosynthesis and contributes to defense and cell death in rice. © 2021 Science China Press
关键词	bZIP transcription factor Cell death HP gene cluster Magnaporthe oryzae Oryza sativa Plant immunity Secondary metabolites
英文关键词	Amino acids; Biochemistry; Biosynthesis; Cell death; Encoding (symbols); Metabolites; Signal encoding; Transcription factors; Biosynthetic gene cluster; bZIP transcription factors; Encodings; Gene clusters; Hydroxycinnamoylputrescine gene cluster; Magnaporthe oryzae; Monocots; Oryza sativa; Plant immunities; Secondary metabolites; Transcription
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207391
作者单位	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; College of Agronomy, Hunan Agricultural University, Changsha, 410128, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China; Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, United States; College of Tropical Crops, Hainan University, Haikou, 572208, China
推荐引用方式 GB/T 7714	Fang H.,Shen S.,Wang D.,et al. A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice[J],2021.
APA	Fang H..,Shen S..,Wang D..,Zhang F..,Zhang C..,...&Ning Y..(2021).A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice.Science Bulletin.
MLA	Fang H.,et al."A monocot-specific hydroxycinnamoylputrescine gene cluster contributes to immunity and cell death in rice".Science Bulletin (2021).