气候变化领域集成服务门户(CCPortal): Single-cell RNA-seq unveils critical regulators of human FOXP3+ regulatory T cell stability

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DOI	10.1016/j.scib.2020.01.002
	Single-cell RNA-seq unveils critical regulators of human FOXP3+ regulatory T cell stability
	Yi G.; Zhao Y.; Xie F.; Zhu F.; Wan Z.; Wang J.; Wang X.; Gao K.; Cao L.; Li X.; Chen C.; Kuang Y.; Qiu X.; Yang H.; Wang J.; Su B.; Chen L.; Zhang W.; Hou Y.; Xu X.; He Y.; Tsun A.; Liu X.; Li B.
发表日期	2020
ISSN	20959273
起始页码	1114
结束页码	1124
卷号	65 期号:13
英文摘要	The heterogeneity and plasticity of T lymphocytes is critical for determining immune response outcomes. Functional regulatory T (Treg) cells are commonly characterized by stable FOXP3 expression and have reported to exhibit heterogeneous phenotypes under inflammatory conditions. However, the interplay between inflammation and Treg cell suppressive activity still remains elusive. Here, we utilized single-cell RNA sequencing to investigate how human Treg cells respond to the pro-inflammatory cytokine interleukin-6 (IL-6). We observed that Treg cells divided into two subpopulations after IL-6 stimulation. TIGIT− unstable Treg cells lost FOXP3 expression and gained an effector-like T cell phenotype, whereas TIGIT+ Treg cells retained robust suppressive function. Single cell transcriptome analysis revealed a spectrum of cellular states of IL-6-stimulated Treg cells and how cytochrome P450 family 1 subfamily A member 1 (CYP1A1) is a crucial regulator of Treg cell suppressive capability and stability. CYP1A1-deficient human Treg cells developed a Th17-like phenotype after IL-6 stimulation. Our findings implicate CYP1A1 as a previously unidentified regulator of Treg cells that may have target potential for clinical application for biotherapies. © 2020 Science China Press
关键词	Cytochrome P450 1A1 Heterogeneity Inflammation Interleukin-6 scRNA-seq Treg cell
英文关键词	Pathology; RNA; T-cells; Cytochrome p450; Heterogeneity; Inflammation; Interleukin-6; scRNA-seq; Treg cells; Cytology
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207272
作者单位	Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; BGI-Shenzhen, Shenzhen, 518083, China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China; School of Mathematical Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Department of Obstetrics and Gynaecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China; Division of Birth Cohort Study, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510006, China; James D. Watson Institute of Genome Sciences, Hangzhou, 310058, China; Biotheus Inc., Zhuhai, 519080, China
推荐引用方式 GB/T 7714	Yi G.,Zhao Y.,Xie F.,et al. Single-cell RNA-seq unveils critical regulators of human FOXP3+ regulatory T cell stability[J],2020,65(13).
APA	Yi G..,Zhao Y..,Xie F..,Zhu F..,Wan Z..,...&Li B..(2020).Single-cell RNA-seq unveils critical regulators of human FOXP3+ regulatory T cell stability.Science Bulletin,65(13).
MLA	Yi G.,et al."Single-cell RNA-seq unveils critical regulators of human FOXP3+ regulatory T cell stability".Science Bulletin 65.13(2020).