气候变化领域集成服务门户(CCPortal): Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice

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DOI	10.1016/j.scib.2020.03.026
	Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice
	Sun L.; Chen R.; Li L.; Yuan B.; Song K.; Pan N.; Cheng T.-L.; Chang S.; Lin K.; He X.; Wu Q.; Xu F.; Qiu Z.; Wang X.
发表日期	2020
ISSN	20959273
起始页码	1192
结束页码	1202
卷号	65 期号:14
英文摘要	Duplications of MECP2-containing genomic segments led to severe autistic symptoms in male. Transgenic mice overexpressing the human MECP2 gene exhibit autistic-like behaviors. Neural circuits underlying social defects in MECP2 transgenic (MECP2-TG) mice remain unknown. To observe neural activity of MECP2-TG mice in vivo, we performed calcium imaging by implantation of microendoscope in the hippocampal CA1 regions of MECP2-TG and wild type (WT) mice. We identified neurons whose activities were tightly associated with social interaction, which activity patterns were compromised in MECP2-TG mice. Strikingly, we rescued the social-related neural activity in CA1 and social defects in MECP2-TG mice by deleting the human MECP2 transgene using the CRISPR/Cas9 method during adulthood. Our data points to the neural circuitry responsible for social interactions and provides potential therapeutic targets for autism in adulthood. © 2020 Science China Press
关键词	Autism CRISPR/Cas9 In vivo calcium imaging MECP2 Social activity
英文关键词	Brain; Defects; Electrophysiology; Neurons; Activity patterns; Calcium imaging; Genomic segments; Neural activity; Neural circuits; Neural ensembles; Social interactions; Therapeutic targets; Mammals
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207183
作者单位	State Key Laboratory of Brain and Cognitive Sciences, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Brain-Intelligence Technology (Shanghai), Institute for Stem Cell and Regeneration, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; Institute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China; The College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China; The College of Life Science, Hebei Normal University, Shijiazhuang, 050016, China; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Wuhan, 430071, China; Advanced Innovation Center for Human Brain Protection, Beijin...
推荐引用方式 GB/T 7714	Sun L.,Chen R.,Li L.,et al. Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice[J],2020,65(14).
APA	Sun L..,Chen R..,Li L..,Yuan B..,Song K..,...&Wang X..(2020).Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice.Science Bulletin,65(14).
MLA	Sun L.,et al."Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice".Science Bulletin 65.14(2020).