气候变化领域集成服务门户(CCPortal): Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation

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DOI	10.1016/j.earscirev.2019.102887
	Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation
	Armbrecht L.H.; Coolen M.J.L.; Lejzerowicz F.; George S.C.; Negandhi K.; Suzuki Y.; Young J.; Foster N.R.; Armand L.K.; Cooper A.; Ostrowski M.; Focardi A.; Stat M.; Moreau J.W.; Weyrich L.S.
发表日期	2019
ISSN	00128252
卷号	196
英文摘要	The study of ancient DNA (aDNA) from sediments (sedaDNA) offers great potential for paleoclimate interpretation, and has recently been applied as a tool to characterise past marine life and environments from deep ocean sediments over geological timescales. Using sedaDNA, palaeo-communities have been detected, including prokaryotes and eukaryotes that do not fossilise, thereby revolutionising the scope of marine micropalaeontological research. However, many studies to date have not reported on the measures taken to prove the authenticity of sedaDNA-derived data from which conclusions are drawn. aDNA is highly fragmented and degraded and extremely sensitive to contamination by non-target environmental DNA. Contamination risks are particularly high on research vessels, drilling ships and platforms, where logistics and facilities do not yet allow for sterile sediment coring, and due consideration needs to be given to sample processing and analysis following aDNA guidelines. This review clarifies the use of aDNA terminology, discusses common pitfalls and highlights the urgency behind adopting new standards for marine sedaDNA research, with a focus on sampling optimisation to facilitate the incorporation of routine sedaDNA research into International Ocean Discovery Program (IODP) operations. Currently available installations aboard drilling ships and platforms are reviewed, improvements suggested, analytical approaches detailed, and the controls and documentation necessary to support the authenticity of aDNA retrieved from deep-sea sediment cores is outlined. Beyond practical considerations, concepts relevant to the study of past marine biodiversity based on sedaDNA, and the applicability of the new guidelines to the study of other contamination-susceptible environments (permafrost and outer space) are discussed. © 2019 Elsevier B.V.
关键词	Ancient DNA Biomarkers Contamination Deep biosphere IODP Marine sediments Mars Phytoplankton Seafloor
英文关键词	biomarker; biosphere; DNA; marine sediment; Mars; paleoecology; phytoplankton; pollutant; seafloor; Eukaryota; Prokaryota
语种	英语
来源期刊	Earth Science Reviews
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/203385
作者单位	Australian Centre for Ancient DNA, School of Biological Sciences, Faculty of Sciences, The University of Adelaide, Adelaide, SA 5005, Australia; Western Australian Organic and Isotope Geochemistry Centre (WA-OIGC), School of Earth and Planetary Sciences, Curtin University, Kent Street, Bentley, WA 6102, Australia; Center for Microbiome Innovation, University of California, San Diego, La Jolla, CA 92093-0403, United States; Department of Earth and Planetary Sciences and MQMarine Research Centre, Macquarie University, North Ryde, NSW 2109, Australia; NSW Office of Environment and Heritage, 10 Valentine Avenue, Parramatta, NSW 2150, Australia; Department of Earth and Planetary Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan; Advanced DNA, Identification and Forensic Facility, The Environment Institute, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia; School of Biological Sciences, Faculty of Sciences, The University of Adelaide, Adelaide, SA 5005, Austr...
推荐引用方式 GB/T 7714	Armbrecht L.H.,Coolen M.J.L.,Lejzerowicz F.,et al. Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation[J],2019,196.
APA	Armbrecht L.H..,Coolen M.J.L..,Lejzerowicz F..,George S.C..,Negandhi K..,...&Weyrich L.S..(2019).Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation.Earth Science Reviews,196.
MLA	Armbrecht L.H.,et al."Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation".Earth Science Reviews 196(2019).