气候变化领域集成服务门户(CCPortal): Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by: Clostridium thermocellum in the presence and absence of continuous in situ ball-milling

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DOI	10.1039/c6ee03748h
	Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by: Clostridium thermocellum in the presence and absence of continuous in situ ball-milling
	Balch M.L.; Holwerda E.K.; Davis M.F.; Sykes R.W.; Happs R.M.; Kumar R.; Wyman C.E.; Lynd L.R.
发表日期	2017
ISSN	17545692
起始页码	1252
结束页码	1261
卷号	10 期号:5
英文摘要	Milling during lignocellulosic fermentation, henceforth referred to as cotreatment, is investigated as an alternative to thermochemical pretreatment as a means of enhancing biological solubilization of lignocellulose. We investigate the impact of milling on soluble substrate fermentation by Clostridium thermocellum with comparison to yeast, document solubilization for fermentation of senescent switchgrass with and without ball milling, and characterize residual solids. Soluble substrate fermentation by C. thermocellum proceeded readily in the presence of continuous ball milling but was completely arrested for yeast. Total fractional carbohydrate solubilization achieved after fermentation of senescent switchgrass by C. thermocellum for 5 days was 0.45 without cotreatment or pretreatment, 0.81 with hydrothermal pretreatment (200 °C, 15 minutes, severity 4.2), and 0.88 with cotreatment. Acetate and ethanol were the main fermentation products, and were produced at similar ratios with and without cotreatment. Analysis of solid residues was undertaken using molecular beam mass spectrometry (PyMBMS) and solid-state nuclear magnetic resonance spectroscopy (NMR) in order to provide insight into changes in plant cell walls during processing via various modes. The structure of lignin present in residual solids remaining after fermentation with cotreatment appeared to change little, with substantially greater changes observed for hydrothermal pretreatment-particularly with respect to formation of C-C bonds. The observation of high solubilization with little apparent modification of the residue is consistent with cotreatment enhancing solubilization primarily by increasing the access of saccharolytic enzymes to the feedstock, and C. thermocellum being able to attack all the major linkages in cellulosic biomass provided that these linkages are accessible. © 2017 The Royal Society of Chemistry.
英文关键词	Cellulose; Clostridium; Fermentation; Lignin; Mass spectrometry; Milling (machining); Molecular beams; Nuclear magnetic resonance spectroscopy; Solubility; Yeast; Cellulosic biomass; Clostridium thermocellum; Fermentation products; Hydrothermal pretreatment; Molecular beam mass spectrometries; Solid state nuclear magnetic resonance spectroscopy; Soluble substrates; Thermochemical pretreatment; Ball milling; bacterium; biomass; carbohydrate; cellulose; fermentation; grass; milling; solubilization; thermochemistry; yeast; Fermentation; Grinding; Lignocellulose; Mass Spectrometers; Nuclear Magnetic Resonance; Clostridium thermocellum; Panicum virgatum
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/190564
作者单位	Thayer School of Engineering, Dartmouth College, Hanover, NH, United States; BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United States; Biosciences Center, National Renewable Energy Laboratory, Golden, CO, United States; National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, United States; Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California, Riverside, Riverside, CA, United States; Chemical and Environmental Engineering Department, University of California, Riverside, CA, United States
推荐引用方式 GB/T 7714	Balch M.L.,Holwerda E.K.,Davis M.F.,et al. Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by: Clostridium thermocellum in the presence and absence of continuous in situ ball-milling[J],2017,10(5).
APA	Balch M.L..,Holwerda E.K..,Davis M.F..,Sykes R.W..,Happs R.M..,...&Lynd L.R..(2017).Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by: Clostridium thermocellum in the presence and absence of continuous in situ ball-milling.Energy & Environmental Science,10(5).
MLA	Balch M.L.,et al."Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by: Clostridium thermocellum in the presence and absence of continuous in situ ball-milling".Energy & Environmental Science 10.5(2017).