气候变化领域集成服务门户(CCPortal): A synthetic biology approach to engineering living photovoltaics

CCPortal

DOI	10.1039/c7ee00282c
	A synthetic biology approach to engineering living photovoltaics
	Schuergers N.; Werlang C.; Ajo-Franklin C.M.; Boghossian A.A.
发表日期	2017
ISSN	17545692
起始页码	1102
结束页码	1115
卷号	10 期号:5
英文摘要	The ability to electronically interface living cells with electron accepting scaffolds is crucial for the development of next-generation biophotovoltaic technologies. Although recent studies have focused on engineering synthetic interfaces that can maximize electronic communication between the cell and scaffold, the efficiency of such devices is limited by the low conductivity of the cell membrane. This review provides a materials science perspective on applying a complementary, synthetic biology approach to engineering membrane-electrode interfaces. It focuses on the technical challenges behind the introduction of foreign extracellular electron transfer pathways in bacterial host cells and past and future efforts to engineer photosynthetic organisms with artificial electron-export capabilities for biophotovoltaic applications. The article highlights advances in engineering protein-based, electron-exporting conduits in a model host organism, E. coli, before reviewing state-of-the-art biophotovoltaic technologies that use both unmodified and bioengineered photosynthetic bacteria with improved electron transport. A thermodynamic analysis is used to propose an energetically feasible pathway for extracellular electron transport in engineered cyanobacteria and identify metabolic bottlenecks amenable to protein engineering techniques. Based on this analysis, an engineered photosynthetic organism expressing a foreign, protein-based electron conduit yields a maximum theoretical solar conversion efficiency of 6-10% without accounting for additional bioengineering optimizations for light-harvesting. © 2017 The Royal Society of Chemistry.
英文关键词	Bacteria; Biology; Efficiency; Electron transport properties; Electrons; Escherichia coli; Interfaces (materials); Proteins; Scaffolds (biology); Thermoanalysis; Electronic communications; Extracellular electron transfer; Photosynthetic bacterias; Photosynthetic organisms; Solar conversion efficiencies; Synthetic interfaces; Technical challenges; Thermo dynamic analysis; Cell engineering; bacterium; bioengineering; cells and cell components; electrical conductivity; equipment; gene expression; membrane; optimization; photovoltaic system; protein; Bacteria (microorganisms); Cyanobacteria; Photobacteria
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/190549
作者单位	Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland; Interschool Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Synthetic Biology Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Schuergers N.,Werlang C.,Ajo-Franklin C.M.,et al. A synthetic biology approach to engineering living photovoltaics[J],2017,10(5).
APA	Schuergers N.,Werlang C.,Ajo-Franklin C.M.,&Boghossian A.A..(2017).A synthetic biology approach to engineering living photovoltaics.Energy & Environmental Science,10(5).
MLA	Schuergers N.,et al."A synthetic biology approach to engineering living photovoltaics".Energy & Environmental Science 10.5(2017).