气候变化领域集成服务门户(CCPortal): A three-species microbial consortium for power generation

CCPortal

DOI	10.1039/c6ee03705d
	A three-species microbial consortium for power generation
	Liu Y.; Ding M.; Ling W.; Yang Y.; Zhou X.; Li B.-Z.; Chen T.; Nie Y.; Wang M.; Zeng B.; Li X.; Liu H.; Sun B.; Xu H.; Zhang J.; Jiao Y.; Hou Y.; Yang H.; Xiao S.; Lin Q.; He X.; Liao W.; Jin Z.; Xie Y.; Zhang B.; Li T.; Lu X.; Li J.; Zhang F.; Wu X.-L.; Song H.; Yuan Y.-J.
发表日期	2017
ISSN	17545692
起始页码	1600
结束页码	1609
卷号	10 期号:7
英文摘要	Design and construction of synthetic microbial consortia is a promising strategy to enhance the performance of bioelectrochemical systems (BESs) and to facilitate practical applications in bioenergy production. According to the design principle of "division-of-labor", we synthesized a three-species microbial consortium for power generation, consisting of engineered Escherichia coli, Bacillus subtilis and Shewanella oneidensis. In this consortium, E. coli digested glucose to produce lactate as a carbon source and an electron donor; B. subtilis produced riboflavin as an electron shuttle; and S. oneidensis served as the exoelectrogen to generate electricity. In return, S. oneidensis oxidized lactate to acetate, which fed E. coli and B. subtilis as the carbon source. Thus, the three species formed a cross-feeding microbial consortium, which performed "better together" for power generation. As a result, glucose (11 mM, total 0.28 g) was converted to electricity for more than 15 days with high energy conversion efficiency (up to 55.7%). The microbial composition and electricity output were stable throughout the operation cycle. Furthermore, the consortium exhibited highly functional robustness to fluctuations in the initial inoculation ratio of the three strains. This system provided new insight into the rational design of more efficient, stable, and robust synthetic microbial consortia applicable in bioenergy and environmental bioremediation. © The Royal Society of Chemistry 2017.
英文关键词	Bacteria; Bacteriology; Bioremediation; Conversion efficiency; Glucose; Bioelectrochemical systems (BESs); Bioenergy productions; Design and construction; Engineered escherichia coli; Generate electricity; High energy conversions; Microbial composition; Shewanella oneidensis; Escherichia coli; bacterium; bioenergy; electricity generation; electrochemical method; energy efficiency; glucose; inoculation; microbial community; Bacillus subtilis; Escherichia coli; Shewanella oneidensis
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/190453
作者单位	Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; SynBio Research Platform, Collaborative Innovation Centre of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin, 300072, China; Team TJU for the International Genetically Engineered Machine Competition (IGEM), Tianjin, 300072, China; School of Chemistry and Environment, Beihang University, Beijing, 100191, China; Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 10087, China
推荐引用方式 GB/T 7714	Liu Y.,Ding M.,Ling W.,et al. A three-species microbial consortium for power generation[J],2017,10(7).
APA	Liu Y..,Ding M..,Ling W..,Yang Y..,Zhou X..,...&Yuan Y.-J..(2017).A three-species microbial consortium for power generation.Energy & Environmental Science,10(7).
MLA	Liu Y.,et al."A three-species microbial consortium for power generation".Energy & Environmental Science 10.7(2017).