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DOI	10.1039/c8ee00774h
	Ultra-rapid rates of water splitting for biohydrogen gas production through: In vitro artificial enzymatic pathways
	Kim E.-J.; Kim J.-E.; Zhang Y.-H.P.J.
发表日期	2018
ISSN	17545692
起始页码	2064
结束页码	2072
卷号	11 期号:8
英文摘要	Unlocking the potential of the hydrogen economy requires breakthroughs in production, storage, distribution, and infrastructure. Here we demonstrate an in vitro artificial enzymatic pathway that can produce hydrogen at extremely high rates by splitting water energized by carbohydrates (e.g., starch). This fifteen hyperthermophilic enzymes pathway comprises ATP-free starch phosphorylation, an NAD-based pentose phosphate pathway, and a biomimetic electron transport chain consisting of a diaphorase, an electron mediator benzyl viologen (BV), and a [NiFe]-hydrogenase, whereas fast electron transfer was facilitated by utilizing a BV-conjugated diaphorase. The highest reaction rate of 530 mmol H2 per L per h was accomplished at 80 °C. Two NAD-conjugated dehydrogenases were further applied to enable nine-day hydrogen production with a total turnover number of NAD of over 100000, along with hyperthermophilic enzymes. This biohydrogen production system characterized by the highest chemical-energy efficiency and an exceptionally-high reaction rate addresses challenges associated with cost-effective, distributed hydrogen production, off-board hydrogen storage, and infrastructure. © 2018 The Royal Society of Chemistry.
英文关键词	Biomimetics; Cost effectiveness; Electron transport properties; Energy efficiency; Enzymes; Hydrogen fuels; Hydrogen storage; Reaction rates; Starch; Bio-hydrogen production; Electron mediator; Electron transport chain; Enzymatic pathways; Fast electron transfer; Pentose phosphate pathway; Starch phosphorylation; [NiFe]-hydrogenase; Hydrogen production; bioactivity; carbohydrate; chemical reaction; electron; energy efficiency; enzyme activity; gas production; hydrogen; hydrolysis; reaction rate
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/190179
作者单位	Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061, United States; C1 Gas Refinery R and D Center, Sogang University, Seoul, 04107, South Korea; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China
推荐引用方式 GB/T 7714	Kim E.-J.,Kim J.-E.,Zhang Y.-H.P.J.. Ultra-rapid rates of water splitting for biohydrogen gas production through: In vitro artificial enzymatic pathways[J],2018,11(8).
APA	Kim E.-J.,Kim J.-E.,&Zhang Y.-H.P.J..(2018).Ultra-rapid rates of water splitting for biohydrogen gas production through: In vitro artificial enzymatic pathways.Energy & Environmental Science,11(8).
MLA	Kim E.-J.,et al."Ultra-rapid rates of water splitting for biohydrogen gas production through: In vitro artificial enzymatic pathways".Energy & Environmental Science 11.8(2018).