气候变化领域集成服务门户(CCPortal): Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

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DOI	10.1039/c8ee01975d
	Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism
	Wegelius A.; Khanna N.; Esmieu C.; Barone G.D.; Pinto F.; Tamagnini P.; Berggren G.; Lindblad P.
发表日期	2018
ISSN	17545692
起始页码	3163
结束页码	3167
卷号	11 期号:11
英文摘要	[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells. © The Royal Society of Chemistry 2018.
英文关键词	Enzymes; Hydrogen fuels; Energy applications; High potential; Hydrogen economy; Metalloenzymes; Photosynthetic microorganisms; Synechocystis pcc 6803; Synthetic enzymes; [FeFe]hydrogenases; Iron compounds; activation energy; biotechnology; catalysis; cell; cyanobacterium; enzyme; enzyme activity; gene expression; hydrogen; metabolism; photosynthesis; Synechocystis sp. PCC 6803
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/190067
作者单位	Microbial Chemistry, Department of Chemistry-Angström, Uppsala University, Box 523, Uppsala, SE-751 20, Sweden; Molecular Biomimetics, Department of Chemistry-Angström, Uppsala University, Box 523, SE-751 20 Uppsala, Sweden; I3S-Instituto de Investigação e Inovação em Saúde, IBMC-Instituto de Biologia Molecular e Celular, Universidade Do Porto, Porto, 4200-135, Portugal; Faculdade de Ciências, Departamento de Biologia, Universidade Do Porto, Porto, 4169-007, Portugal
推荐引用方式 GB/T 7714	Wegelius A.,Khanna N.,Esmieu C.,et al. Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism[J],2018,11(11).
APA	Wegelius A..,Khanna N..,Esmieu C..,Barone G.D..,Pinto F..,...&Lindblad P..(2018).Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism.Energy & Environmental Science,11(11).
MLA	Wegelius A.,et al."Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism".Energy & Environmental Science 11.11(2018).