气候变化领域集成服务门户(CCPortal): Cross-species recognition of SARS-CoV-2 to bat ACE2

CCPortal

DOI	10.1073/pnas.2020216118
	Cross-species recognition of SARS-CoV-2 to bat ACE2
	Liu K.; Tan S.; Niu S.; Wang J.; Wu L.; Sun H.; Zhang Y.; Pan X.; Qu X.; Du P.; Meng Y.; Jia Y.; Chen Q.; Deng C.; Yan J.; Wang H.-W.; Wang Q.; Qi J.; Gao G.F.
发表日期	2021
ISSN	00278424
卷号	118 期号:1
英文摘要	The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as a major threat to global health. Although varied SARS-CoV-2-related coronaviruses have been isolated from bats and SARS-CoV-2 may infect bat, the structural basis for SARS-CoV-2 to utilize the human receptor counterpart bat angiotensin-converting enzyme 2 (bACE2) for virus infection remains less understood. Here, we report that the SARS-CoV-2 spike protein receptor binding domain (RBD) could bind to bACE2 from Rhinolophus macrotis (bACE2- Rm) with substantially lower affinity compared with that to the human ACE2 (hACE2), and its infectivity to host cells expressing bACE2- Rm was confirmed with pseudotyped SARS-CoV-2 virus and SARS-CoV-2 wild virus. The structure of the SARS-CoV-2 RBD with the bACE2-Rm complex was determined, revealing a binding mode similar to that of hACE2. The analysis of binding details between SARS-CoV-2 RBD and bACE2-Rm revealed that the interacting network involving Y41 and E42 of bACE2-Rm showed substantial differences with that to hACE2. Bats have extensive species diversity and the residues for RBD binding in bACE2 receptor varied substantially among different bat species. Notably, the Y41H mutant, which exists in many bats, attenuates the binding capacity of bACE2-Rm, indicating the central roles of Y41 in the interaction network. These findings would benefit our understanding of the potential infection of SARS-CoV-2 in varied species of bats. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	ACE2; COVID-19; RBD; Rhinolophus macrotis; SARS-CoV-2
语种	英语
scopus关键词	angiotensin converting enzyme 2; homodimer; virus spike protein; ACE2 protein, human; protein binding; animal cell; Article; binding affinity; controlled study; coronavirus disease 2019; host cell; mouse; nonhuman; priority journal; protein binding; protein expression; protein protein interaction; protein structure; receptor binding; Rhinolophus; Rhinolophus macrotis; Severe acute respiratory syndrome coronavirus 2; species diversity; virus infectivity; amino acid substitution; animal; bat; chemistry; epidemiology; genetics; HEK293 cell line; human; metabolism; missense mutation; pandemic; protein domain; species difference; virology; Amino Acid Substitution; Angiotensin-Converting Enzyme 2; Animals; Chiroptera; COVID-19; HEK293 Cells; Humans; Mutation, Missense; Pandemics; Protein Binding; Protein Domains; SARS-CoV-2; Species Specificity
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181160
作者单位	Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Faculty of Health Sciences, University of Macau, Macau SAR, 999078, Macau; College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, 030801, China; Ministry of Education Key Laboratory of Protein Sciences, Tsinghua-Peking Joint Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center of Biological Structures, School of Life Sciences, Tsinghua University, Beijing, 100084, China; Chinese Academy of Sciences Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; Inst...
推荐引用方式 GB/T 7714	Liu K.,Tan S.,Niu S.,et al. Cross-species recognition of SARS-CoV-2 to bat ACE2[J],2021,118(1).
APA	Liu K..,Tan S..,Niu S..,Wang J..,Wu L..,...&Gao G.F..(2021).Cross-species recognition of SARS-CoV-2 to bat ACE2.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA	Liu K.,et al."Cross-species recognition of SARS-CoV-2 to bat ACE2".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).