气候变化领域集成服务门户(CCPortal): MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato

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DOI	10.1073/pnas.2022788118
	MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato
	Zhu L.; Li B.; Wu L.; Li H.; Wang Z.; Wei X.; Ma B.; Zhang Y.; Ma F.; Ruan Y.-L.; Li M.
发表日期	2021
ISSN	00278424
卷号	118 期号:1
英文摘要	Sugar transport across tonoplasts is essential for maintaining cellular sugar homeostasis and metabolic balance in plant cells. It remains unclear, however, how this process is regulated among different classes of sugar transporters. Here, we identified a tonoplast H+/glucose symporter, MdERDL6-1, from apples, which was highly expressed in fruits and exhibited expression patterns similar to those of the tonoplast H+/sugar antiporters MdTST1 and MdTST2. Overexpression of MdERDL6-1 unexpectedly increased not only glucose (Glc) concentration but also that of fructose (Fru) and sucrose (Suc) in transgenic apple and tomato leaves and fruits. RNA sequencing (RNA-seq) and expression analyses showed an upregulation of TST1 and TST2 in the transgenic apple and tomato lines overexpressing MdERDL6-1. Further studies established that the increased sugar concentration in the transgenic lines correlated with up-regulation of TST1 and TST2 expression. Suppression or knockout of SlTST1 and SlTST2 in the MdERDL6-1-overexpressed tomato background reduced or abolished the positive effect of MdERDL6-1 on sugar accumulation, respectively. The findings demonstrate a regulation of TST1 and TST2 by MdERDL6-1, inwhich Glc exported by MdERDL6-1 from vacuole up-regulates TST1 and TST2 to import sugars from cytosol to vacuole for accumulation to high concentrations. The results provide insight into the regulatory mechanism of sugar accumulation in vacuoles mediated by the coordinated action of two classes of tonoplast sugar transporters. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cytosolic sugar signaling; H+/glucose symporter; H+/sugar antiporter; Intracellular communication; Sugar transport
语种	英语
scopus关键词	glucose; messenger RNA; sucrose; sugar; Article; bioaccumulation; cellular distribution; controlled study; cytosol; fruit; gene control; gene expression profiling; gene knockout; gene silencing; glucose transport; Malus domestica; MdERDL6 1 gene; molecular cloning; nonhuman; plant gene; plant leaf; priority journal; promoter region; real time polymerase chain reaction; RNA sequencing; tomato; transgenic plant; TST1 gene; TST2 gene; upregulation; Western blotting
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181159
作者单位	State Key Laboratory of Crop Stress Biology in Arid Areas, Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China; Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Canberra, ACT 2601, Australia; Hybrid Rapeseed Research Center of Shaanxi Province, Yangling, Shaanxi, 712100, China; School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW 2308, Australia
推荐引用方式 GB/T 7714	Zhu L.,Li B.,Wu L.,et al. MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato[J],2021,118(1).
APA	Zhu L..,Li B..,Wu L..,Li H..,Wang Z..,...&Li M..(2021).MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA	Zhu L.,et al."MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).