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| DOI | 10.1073/pnas.2020454118 |
| A genome-wide microRNA screen identifies the microRNA-183/96/182 cluster as a modulator of circadian rhythms | |
| Zhou L.; Miller C.; Miraglia L.J.; Romero A.; Mure L.S.; Panda S.; Kay S.A. | |
| 发表日期 | 2021 |
| ISSN | 00278424 |
| 卷号 | 118期号:1 |
| 英文摘要 | The regulatory mechanisms of circadian rhythms have been studied primarily at the level of the transcription-translation feedback loops of protein-coding genes. Regulatory modules involving noncoding RNAs are less thoroughly understood. In particular, emerging evidence has revealed the important role of microRNAs (miRNAs) in maintaining the robustness of the circadian system. To identify miRNAs that have the potential to modulate circadian rhythms, we conducted a genome-wide miRNA screen using U2OS luciferase reporter cells. Among 989 miRNAs in the library, 120 changed the period length in a dose-dependent manner. We further validated the circadian regulatory function of an miRNA cluster, miR-183/96/182, both in vitro and in vivo. We found that all three members of this miRNA cluster can modulate circadian rhythms. Particularly, miR-96 directly targeted a core circadian clock gene, PER2. The knockout of the miR-183/96/182 cluster in mice showed tissue-specific effects on circadian parameters and altered circadian rhythms at the behavioral level. This study identified a large number of miRNAs, including the miR-183/96/182 cluster, as circadian modulators. We provide a resource for further understanding the role of miRNAs in the circadian network and highlight the importance of miRNAs as a genome-wide layer of circadian clock regulation. © 2021 National Academy of Sciences. All rights reserved. |
| 英文关键词 | Circadian rhythms; Genome-wide screen; MiR-183/96/182 cluster; MiRNA |
| 语种 | 英语 |
| scopus关键词 | luciferase; microRNA; microRNA 182; microRNA 183; microRNA 96; PER2 protein; unclassified drug; Article; circadian rhythm; controlled study; gene cluster; gene function; gene library; gene number; human; human cell; in vitro study; in vivo study; medical parameters; PER2 gene; priority journal; regulatory mechanism; RNA analysis; tissue specificity; U2OS cell line; validation study |
| 来源期刊 | Proceedings of the National Academy of Sciences of the United States of America
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| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/181157 |
| 作者单位 | Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, United States; Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, United States; Salk Institute for Biological Studies, San Diego, CA 92037, United States |
| 推荐引用方式 GB/T 7714 | Zhou L.,Miller C.,Miraglia L.J.,et al. A genome-wide microRNA screen identifies the microRNA-183/96/182 cluster as a modulator of circadian rhythms[J],2021,118(1). |
| APA | Zhou L..,Miller C..,Miraglia L.J..,Romero A..,Mure L.S..,...&Kay S.A..(2021).A genome-wide microRNA screen identifies the microRNA-183/96/182 cluster as a modulator of circadian rhythms.Proceedings of the National Academy of Sciences of the United States of America,118(1). |
| MLA | Zhou L.,et al."A genome-wide microRNA screen identifies the microRNA-183/96/182 cluster as a modulator of circadian rhythms".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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