气候变化领域集成服务门户(CCPortal): Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration

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DOI	10.1073/PNAS.1904338118
	Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration
	Kato M.; Kolotuev I.; Cunha A.; Gharib S.; Sternberg P.W.
发表日期	2021
ISSN	00278424
卷号	118 期号:1
英文摘要	Acetylcholine (ACh) promotes various cell migrations in vitro, but there are few investigations into this nonsynaptic role of ACh signaling in vivo. Here we investigate the function of a muscarinic receptor on an epithelial cell migration in Caenorhabditis elegans. We show that the migratory gonad leader cell, the linker cell (LC), uses an M1/M3/M5-like muscarinic ACh receptor GAR-3 to receive extrasynaptic ACh signaling from cholinergic neurons for its migration. Either the loss of the GAR-3 receptor in the LC or the inhibition of ACh release from cholinergic neurons resulted in migratory path defects. The overactivation of the GAR-3 muscarinic receptor caused the LC to reverse its orientation through its downstream effectors Gαq/egl-30, PLCβ/egl-8, and TRIO/unc-73. This reversal response only occurred in the fourth larval stage, which corresponds to the developmental time when the GAR-3::yellow fluorescent protein receptor in the membrane relocalizes from a uniform to an asymmetric distribution. These findings suggest a role for the GAR-3 muscarinic receptor in determining the direction of LC migration. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Acetylcholine; Cell migration; Muscarinic receptor
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181133
作者单位	Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States; Electron Microscopy Facility, University of Lausanne, Quartier Sorge-Biophore, Lausanne, CH-1015, Switzerland; Center for Advanced Methods in Biological Image Analysis, California Institute of Technology, Pasadena, CA 91125, United States
推荐引用方式 GB/T 7714	Kato M.,Kolotuev I.,Cunha A.,et al. Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration[J],2021,118(1).
APA	Kato M.,Kolotuev I.,Cunha A.,Gharib S.,&Sternberg P.W..(2021).Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA	Kato M.,et al."Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).