气候变化领域集成服务门户(CCPortal): ROS and hypoxia signaling regulate periodic metabolic arousal during insect dormancy to coordinate glucose, amino acid, and lipid metabolism

CCPortal

DOI	10.1073/PNAS.2017603118
	ROS and hypoxia signaling regulate periodic metabolic arousal during insect dormancy to coordinate glucose, amino acid, and lipid metabolism
	Chen C.; Mahar R.; Merritt M.E.; Denlinger D.L.; Hahn D.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:1
英文摘要	Metabolic suppression is a hallmark of animal dormancy that promotes overall energy savings. Some diapausing insects and some mammalian hibernators have regular cyclic patterns of substantial metabolic depression alternating with periodic arousal where metabolic rates increase dramatically. Previous studies, largely in mammalian hibernators, have shown that periodic arousal is driven by an increase in aerobic mitochondrial metabolism and that many molecules related to energy metabolism fluctuate predictably across periodic arousal cycles. However, it is still not clear how these rapid metabolic shifts are regulated. We first found that diapausing flesh fly pupae primarily use anaerobic glycolysis during metabolic depression but engage in aerobic respiration through the tricarboxylic acid cycle during periodic arousal. Diapausing pupae also clear anaerobic by-products and regenerate many metabolic intermediates depleted in metabolic depression during arousal, consistent with patterns in mammalian hibernators. We found that decreased levels of reactive oxygen species (ROS) induced metabolic arousal and elevated ROS extended the duration of metabolic depression. Our data suggest ROS regulates the timing of metabolic arousal by changing the activity of two critical metabolic enzymes, pyruvate dehydrogenase and carnitine palmitoyltransferase I by modulating the levels of hypoxia inducible transcription factor (HIF) and phosphorylation of adenosine 5′-monophosphate-activated protein kinase (AMPK). Our study shows that ROS signaling regulates periodic arousal in our insect diapasue system, suggesting the possible importance ROS for regulating other types of of metabolic cycles in dormancy as well. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Diapause; Hibernation; Hypoxia signaling; Periodic arousal; ROS
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181118
作者单位	Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611-0620, United States; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610-0245, United States; Department of Entomology, 300 Aronoff Laboratory, Ohio State University, Columbus, OH 43210, United States; Department of Evolution, Ecology, and Organismal Biology, Ohio State University, 300 Aronoff Laboratory, Columbus, OH 43210, United States; Genetics Institute, University of Florida, Gainesville, FL 32610-3610, United States
推荐引用方式 GB/T 7714	Chen C.,Mahar R.,Merritt M.E.,et al. ROS and hypoxia signaling regulate periodic metabolic arousal during insect dormancy to coordinate glucose, amino acid, and lipid metabolism[J],2021,118(1).
APA	Chen C.,Mahar R.,Merritt M.E.,Denlinger D.L.,&Hahn D.A..(2021).ROS and hypoxia signaling regulate periodic metabolic arousal during insect dormancy to coordinate glucose, amino acid, and lipid metabolism.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA	Chen C.,et al."ROS and hypoxia signaling regulate periodic metabolic arousal during insect dormancy to coordinate glucose, amino acid, and lipid metabolism".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).