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DOI	10.1073/pnas.2021785118
	Structure of SARS-cov-2 ORF8, a rapidly evolving immune evasion protein
	Flower T.G.; Buffalo C.Z.; Hooy R.M.; Allaire M.; Ren X.; Hurley J.H.
发表日期	2021
ISSN	00278424
卷号	118 期号:2
英文摘要	The molecular basis for the severity and rapid spread of the COVID-19 disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely unknown. ORF8 is a rapidly evolving accessory protein that has been proposed to interfere with immune responses. The crystal structure of SARS-CoV-2 ORF8 was determined at 2.04-Å resolution by X-ray crystallography. The structure reveals a ∼60-residue core similar to SARS-CoV-2 ORF7a, with the addition of two dimerization interfaces unique to SARS-CoV-2 ORF8. A covalent disulfide-linked dimer is formed through an N-terminal sequence specific to SARS-CoV-2, while a separate noncovalent interface is formed by another SARS-CoV-2−specific sequence, 73YIDI76. Together, the presence of these interfaces shows how SARS-CoV-2 ORF8 can form unique large-scale assemblies not possible for SARS-CoV, potentially mediating unique immune suppression and evasion activities. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	COVID-19; SARS-cov-2; X-ray crystallography
语种	英语
scopus关键词	ORF7a protein; ORF8 protein; unclassified drug; viral protein; ORF8 protein, SARS-CoV-2; viral protein; amino terminal sequence; Article; beta sheet; crystal structure; dimerization; disulfide bond; immune evasion; nonhuman; priority journal; protein secondary structure; protein structure; sequence alignment; Severe acute respiratory syndrome coronavirus 2; X ray crystallography; chemical structure; chemistry; immune evasion; molecular evolution; Evolution, Molecular; Immune Evasion; Molecular Structure; SARS-CoV-2; Viral Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181089
作者单位	Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, United States; California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, United States; Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Flower T.G.,Buffalo C.Z.,Hooy R.M.,et al. Structure of SARS-cov-2 ORF8, a rapidly evolving immune evasion protein[J],2021,118(2).
APA	Flower T.G.,Buffalo C.Z.,Hooy R.M.,Allaire M.,Ren X.,&Hurley J.H..(2021).Structure of SARS-cov-2 ORF8, a rapidly evolving immune evasion protein.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA	Flower T.G.,et al."Structure of SARS-cov-2 ORF8, a rapidly evolving immune evasion protein".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).