气候变化领域集成服务门户(CCPortal): Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration

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DOI	10.1073/pnas.2009539118
	Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration
	Cao Z.; Meng Y.; Gong F.; Xu Z.; Liu F.; Fang M.; Zou L.; Liao X.; Wang X.; Luo L.; Li X.; Lu H.
发表日期	2021
ISSN	00278424
卷号	118 期号:2
英文摘要	Planarian flatworms regenerate their heads and tails from anterior or posterior wounds and this regenerative blastema polarity is controlled by Wnt/β-catenin signaling. It is well known that a regeneration blastema of appendages of vertebrates such as fish and amphibians grows distally. However, it remains unclear whether a regeneration blastema in vertebrate appendages can grow proximally. Here, we show that a regeneration blastema in zebrafish fins can grow proximally along the proximodistal axis by calcineurin inhibition. We used fin excavation in adult zebrafish to observe unidirectional regeneration from the anterior cut edge (ACE) to the posterior cut edge (PCE) of the cavity and this unidirectional regeneration polarity occurs as the PCE fails to build blastemas. Furthermore, we found that calcineurin activities in the ACE were greater than in the PCE. Calcineurin inhibition induced PCE blastemas, and calcineurin hyperactivation suppressed fin regeneration. Collectively, these findings identify calcineurin as a molecular switch to specify the PCE blastema of the proximodistal axis and regeneration polarity in zebrafish fin. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Calcineurin; Fin regeneration; Polarity; Zebrafish
语种	英语
scopus关键词	calcineurin; fk 506 binding protein; retinoic acid; adult; anatomical concepts; anterior cut edge; Article; blastema; embryo polarity; enzyme activation; enzyme activity; enzyme inhibition; fin (organ); nonhuman; posterior cut edge; priority journal; protein function; proximal-distal patterning; signal transduction; tissue regeneration; zebra fish
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181065
作者单位	Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs, Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji'an, Jiangxi, 343009, China; Center for Drug Screening and Research, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, Jiangxi, 341000, China; School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Institute of Developmental Biology and Regenerative Medicine, Southwest University, Beibei, Chongqing, 400715, China
推荐引用方式 GB/T 7714	Cao Z.,Meng Y.,Gong F.,et al. Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration[J],2021,118(2).
APA	Cao Z..,Meng Y..,Gong F..,Xu Z..,Liu F..,...&Lu H..(2021).Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA	Cao Z.,et al."Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).