气候变化领域集成服务门户(CCPortal): Minimal gene set from Sinorhizobium (Ensifer) meliloti pSymA required for efficient symbiosis with Medicago

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DOI	10.1073/pnas.2018015118
	Minimal gene set from Sinorhizobium (Ensifer) meliloti pSymA required for efficient symbiosis with Medicago
	Geddes B.A.; Kearsley J.V.S.; Huang J.; Zamani M.; Muhammed Z.; Sather L.; Panchal A.K.; diCenzo G.C.; Finan T.M.
发表日期	2021
ISSN	00278424
卷号	118 期号:2
英文摘要	Reduction of N2 gas to ammonia in legume root nodules is a key component of sustainable agricultural systems. Root nodules are the result of a symbiosis between leguminous plants and bacteria called rhizobia. Both symbiotic partners play active roles in establishing successful symbiosis and nitrogen fixation: while root nodule development is mostly controlled by the plant, the rhizobia induce nodule formation, invade, and perform N2 fixation once inside the plant cells. Many bacterial genes involved in the rhizobia-legume symbiosis are known, and there is much interest in engineering the symbiosis to include major nonlegume crops such as corn, wheat, and rice. We sought to identify and combine a minimal bacterial gene complement necessary and sufficient for symbiosis. We analyzed a model rhizobium, Sinorhizobium (Ensifer) meliloti, using a background strain in which the 1.35-Mb symbiotic megaplasmid pSymA was removed. Three regions representing 162 kb of pSymA were sufficient to recover a complete N2-fixing symbiosis with alfalfa, and a targeted assembly of this gene complement achieved high levels of symbiotic N2 fixation. The resulting gene set contained just 58 of 1,290 pSymA protein-coding genes. To generate a platform for future synthetic manipulation, the minimal symbiotic genes were reorganized into three discrete nod, nif, and fix modules. These constructs will facilitate directed studies toward expanding the symbiosis to other plant partners. They also enable forward-type approaches to identifying genetic components that may not be essential for symbiosis, but which modulate the rhizobium's competitiveness for nodulation and the effectiveness of particular rhizobia-plant symbioses. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Nitrogen fixation; Rhizobia; Root nodule; Symbiosis; Synthetic biology
语种	英语
scopus关键词	alfalfa; article; bacterial gene; legume; nitrogen fixation; nonhuman; Rhizobiaceae; rice; Sinorhizobium meliloti; synthetic biology; wheat
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181064
作者单位	Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada
推荐引用方式 GB/T 7714	Geddes B.A.,Kearsley J.V.S.,Huang J.,et al. Minimal gene set from Sinorhizobium (Ensifer) meliloti pSymA required for efficient symbiosis with Medicago[J],2021,118(2).
APA	Geddes B.A..,Kearsley J.V.S..,Huang J..,Zamani M..,Muhammed Z..,...&Finan T.M..(2021).Minimal gene set from Sinorhizobium (Ensifer) meliloti pSymA required for efficient symbiosis with Medicago.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA	Geddes B.A.,et al."Minimal gene set from Sinorhizobium (Ensifer) meliloti pSymA required for efficient symbiosis with Medicago".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).