气候变化领域集成服务门户(CCPortal): Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament

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DOI	10.1073/pnas.2022826118
	Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament
	Zheng W.; Peña A.; Ilangovan A.; Clark J.N.-B.; Frankel G.; Egelman E.H.; Costa T.R.D.
发表日期	2021
ISSN	00278424
卷号	118 期号:2
英文摘要	Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC) utilize a macromolecular type III secretion system (T3SS) to inject effector proteins into eukaryotic cells. This apparatus spans the inner and outer bacterial membranes and includes a helical needle protruding into the extracellular space. Thus far observed only in EPEC and EHEC and not found in other pathogenic Gram-negative bacteria that have a T3SS is an additional helical filament made by the EspA protein that forms a long extension to the needle, mediating both attachment to eukaryotic cells and transport of effector proteins through the intestinal mucus layer. Here, we present the structure of the EspA filament from EPEC at 3.4 Å resolution. The structure reveals that the EspA filament is a right-handed 1-start helical assembly with a conserved lumen architecture with respect to the needle to ensure the seamless transport of unfolded cargos en route to the target cell. This functional conservation is despite the fact that there is little apparent overall conservation at the level of sequence or structure with the needle. We also unveil the molecular details of the immunodominant EspA epitope that can now be exploited for the rational design of epitope display systems. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Attaching/effacing pathogens; Bacterial pathogenesis; Intestinal epithelium
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181038
作者单位	Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22903, United States; MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College, London, SW7 2AZ, United Kingdom; School of Biological and Chemical Sciences, Queen Mary University of London, London, E1 4NS, United Kingdom
推荐引用方式 GB/T 7714	Zheng W.,Peña A.,Ilangovan A.,et al. Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament[J],2021,118(2).
APA	Zheng W..,Peña A..,Ilangovan A..,Clark J.N.-B..,Frankel G..,...&Costa T.R.D..(2021).Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA	Zheng W.,et al."Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).