气候变化领域集成服务门户(CCPortal): Physiological role of the 3′IgH CBEs super-anchor in antibody class switching

CCPortal

DOI	10.1073/PNAS.2024392118
	Physiological role of the 3′IgH CBEs super-anchor in antibody class switching
	Zhang X.; Yoon H.S.; Chapdelaine-Williams A.M.; Kyritsis N.; Alt F.W.
发表日期	2021
ISSN	00278424
卷号	118 期号:3
英文摘要	IgH class switch recombination (CSR) replaces Cμ constant region (CH) exons with one of six downstream CHs by joining transcription-targeted double-strand breaks (DSBs) in the Cμ switch (S) region to DSBs in a downstream S region. Chromatin loop extrusion underlies fundamental CSR mechanisms including 3′IgH regulatory region (3′IgHRR)-mediated S region transcription, CSR center formation, and deletional CSR joining. There are 10 consecutive CTCF-binding elements (CBEs) downstream of the 3′IgHRR, termed the “3′IgH CBEs.” Prior studies showed that deletion of eight 3′IgH CBEs did not detectably affect CSR. Here, we report that deletion of all 3′IgH CBEs impacts, to varying degrees, germline transcription and CSR of upstream S regions, except that of Sγ1. Moreover, deletion of all 3′IgH CBEs rendered the 6-kb region just downstream highly transcribed and caused sequences within to be aligned with Sμ, broken, and joined to form aberrant CSR rearrangements. These findings implicate the 3′IgH CBEs as critical insulators for focusing loop extrusion-mediated 3′IgHRR transcriptional and CSR activities on upstream CH locus targets. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	3'IgH CBEs; Chromatin loop extrusion; Class switch recombination; Promoter competition
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181015
作者单位	Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115, United States; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, United States; Department of Genetics, Harvard Medical School, Boston, MA 02115, United States
推荐引用方式 GB/T 7714	Zhang X.,Yoon H.S.,Chapdelaine-Williams A.M.,等. Physiological role of the 3′IgH CBEs super-anchor in antibody class switching[J],2021,118(3).
APA	Zhang X.,Yoon H.S.,Chapdelaine-Williams A.M.,Kyritsis N.,&Alt F.W..(2021).Physiological role of the 3′IgH CBEs super-anchor in antibody class switching.Proceedings of the National Academy of Sciences of the United States of America,118(3).
MLA	Zhang X.,et al."Physiological role of the 3′IgH CBEs super-anchor in antibody class switching".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021).