气候变化领域集成服务门户(CCPortal): Regulation of a subset of release-ready vesicles by the presynaptic protein Mover

CCPortal

DOI	10.1073/pnas.2022551118
	Regulation of a subset of release-ready vesicles by the presynaptic protein Mover
	Pofantis E.; Neher E.; Dresbach T.
发表日期	2021
ISSN	00278424
卷号	118 期号:3
英文摘要	Neurotransmitter release occurs by regulated exocytosis from synaptic vesicles (SVs). Evolutionarily conserved proteins mediate the essential aspects of this process, including the membrane fusion step and priming steps that make SVs release-competent. Unlike the proteins constituting the core fusion machinery, the SV protein Mover does not occur in all species and all synapses. Its restricted expression suggests that Mover may modulate basic aspects of transmitter release and short-term plasticity. To test this hypothesis, we analyzed synaptic transmission electrophysiologically at the mouse calyx of Held synapse in slices obtained from wild-type mice and mice lacking Mover. Spontaneous transmission was unaffected, indicating that the basic release machinery works in the absence of Mover. Evoked release and vesicular release probability were slightly reduced, and the paired pulse ratio was increased in Mover knockout mice. To explore whether Mover’s role is restricted to certain subpools of SVs, we analyzed our data in terms of two models of priming. A model assuming two SV pools in parallel showed a reduced release probability of so-called “superprimed vesicles” while “normally primed” ones were unaffected. For the second model, which holds that vesicles transit sequentially from a loosely docked state to a tightly docked state before exocytosis, we found that knocking out Mover selectively decreased the release probability of tight state vesicles. These results indicate that Mover regulates a subclass of primed SVs in the mouse calyx of Held. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Mover; Neurotransmission; Presynaptic plasticity; Synaptic vesicles; Vesicle priming
语种	英语
scopus关键词	animal experiment; article; exocytosis; kidney calyx; knockout gene; knockout mouse; male; mouse; neurotransmitter release; nonhuman; probability; synapse vesicle; synaptic transmission; wild type mouse
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180985
作者单位	Institute of Anatomy and Embryology, University Medical Center Göttingen, Georg-August University of Göttingen, Göttingen, 37075, Germany; Emeritus Laboratory of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen, 37077, Germany; Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells, University of Göttingen, Göttingen, Germany
推荐引用方式 GB/T 7714	Pofantis E.,Neher E.,Dresbach T.. Regulation of a subset of release-ready vesicles by the presynaptic protein Mover[J],2021,118(3).
APA	Pofantis E.,Neher E.,&Dresbach T..(2021).Regulation of a subset of release-ready vesicles by the presynaptic protein Mover.Proceedings of the National Academy of Sciences of the United States of America,118(3).
MLA	Pofantis E.,et al."Regulation of a subset of release-ready vesicles by the presynaptic protein Mover".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021).