气候变化领域集成服务门户(CCPortal): Phylogenomic fingerprinting of tempo and functions of horizontal gene transfer within ochrophytes

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DOI	10.1073/PNAS.2009974118
	Phylogenomic fingerprinting of tempo and functions of horizontal gene transfer within ochrophytes
	Dorrell R.G.; Villain A.; Perez-Lamarque B.; Kerdrel G.A.D.; McCallum G.; Watson A.K.; Ait-Mohamed O.; Alberti A.; Corre E.; Frischkorn K.R.; Karlusicha J.J.P.; Pelletier E.; Morlon H.; Bowler C.; Blanc G.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	Horizontal gene transfer (HGT) is an important source of novelty in eukaryotic genomes. This is particularly true for the ochrophytes, a diverse and important group of algae. Previous studies have shown that ochrophytes possess a mosaic of genes derived from bacteria and eukaryotic algae, acquired through chloroplast endosymbiosis and from HGTs, although understanding of the time points and mechanisms underpinning these transfers has been restricted by the depth of taxonomic sampling possible. We harness an expanded set of ochrophyte sequence libraries, alongside automated and manual phylogenetic annotation, in silico modeling, and experimental techniques, to assess the frequency and functions of HGT across this lineage. Through manual annotation of thousands of single-gene trees, we identify continuous bacterial HGT as the predominant source of recently arrived genes in the model diatom Phaeodactylum tricornutum. Using a large-scale automated dataset, a multigene ochrophyte reference tree, and mathematical reconciliation of gene trees, we note a probable elevation of bacterial HGTs at foundational points in diatom evolution, following their divergence from other ochrophytes. Finally, we demonstrate that throughout ochrophyte evolutionary history, bacterial HGTs have been enriched in genes encoding secreted proteins. Our study provides insights into the sources and frequency of HGTs, and functional contributions that HGT has made to algal evolution. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Mmetsp; Ornithine-urea cycle; Phylogenomics; Rnaseq; Stramenopile
语种	英语
scopus关键词	article; computer model; horizontal gene transfer; library; nonhuman; Phaeodactylum tricornutum; phylogenomics; RNA sequencing; urea cycle
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180933
作者单位	Institut de Biologie de l'École Normale Supérieure, Ecole Normale Supérieure, Université Paris Sciences et Lettres, CNRS, Inserm, Paris, 75005, France; Institut de Recherche Pour le Développement (IRD), Aix Marseille University, Universite de Toulon, Cnrs, Marseille, 13288, France; Institut de Systématique, Evolution, Biodiversité (ISYEB), Museúm National d'Histoire Naturelle, CNRS, Sorbonne Université, École Pratique des Hautes Études (EPHE), Paris, 75005, France; Department of Biology, Concordia University, Montreal, QC H3G 1M8, Canada; Metabolic Genomics, Genoscope, Institut de Biologie Francois Jacob, Université Evry, Université Paris-Saclay, Evry, France; Université Pierre et Marie Curie Paris 06, Station Biologique de Roscoff, Paris, 75005, France; Research Federation for Study of Global Ocean Systems Ecology and Evolution, FR2022/Tara Oceans Global Ocean Systems Ecology and Evolution, Paris, 75016, France; Bioaster, Lyon, 69007, France; Centre d'Études Prospectives et d'Informations Internationale...
推荐引用方式 GB/T 7714	Dorrell R.G.,Villain A.,Perez-Lamarque B.,et al. Phylogenomic fingerprinting of tempo and functions of horizontal gene transfer within ochrophytes[J],2021,118(4).
APA	Dorrell R.G..,Villain A..,Perez-Lamarque B..,Kerdrel G.A.D..,McCallum G..,...&Blanc G..(2021).Phylogenomic fingerprinting of tempo and functions of horizontal gene transfer within ochrophytes.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Dorrell R.G.,et al."Phylogenomic fingerprinting of tempo and functions of horizontal gene transfer within ochrophytes".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).