气候变化领域集成服务门户(CCPortal): Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome

CCPortal

DOI	10.1073/pnas.2014498118
	Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome
	Nodelman I.M.; Shen Z.; Levendosky R.F.; Bowman G.D.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	Chromatin remodelers are ATP (adenosine triphosphate)-powered motors that reposition nucleosomes throughout eukaryotic chromosomes. Remodelers possess autoinhibitory elements that control the direction of nucleosome sliding, but underlying mechanisms of inhibition have been unclear. Here, we show that autoinhibitory elements of the yeast Chd1 remodeler block nucleosome sliding by preventing initiation of twist defects. We show that two autoinhibitory elements—the chromodomains and bridge—reinforce each other to block sliding when the DNA-binding domain is not bound to entry-side DNA. Our data support a model where the chromodomains and bridge target nucleotide-free and ADP-bound states of the ATPase motor, favoring a partially disengaged state of the ATPase motor on the nucleosome. By bypassing distortions of nucleosomal DNA prior to ATP binding, we propose that autoinhibitory elements uncouple the ATP binding/hydrolysis cycle from DNA translocation around the histone core. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Allostery; Asymmetric histone core; Chromatin remodeling; Nucleosome sliding; Snf2 ATPase
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180878
作者单位	Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, United States
推荐引用方式 GB/T 7714	Nodelman I.M.,Shen Z.,Levendosky R.F.,et al. Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome[J],2021,118(4).
APA	Nodelman I.M.,Shen Z.,Levendosky R.F.,&Bowman G.D..(2021).Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Nodelman I.M.,et al."Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).