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DOI	10.1073/pnas.2009959118
	On the adhesion–velocity relation and length adaptation of motile cells on stepped fibronectin lanes
	Schreiber C.; Amiri B.; Heyn J.C.J.; Rädler J.O.; Falcke M.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	The biphasic adhesion–velocity relation is a universal observation in mesenchymal cell motility. It has been explained by adhesion-promoted forces pushing the front and resisting motion at the rear. Yet, there is little quantitative understanding of how these forces control cell velocity. We study motion of MDA-MB-231 cells on microlanes with fields of alternating Fibronectin densities to address this topic and derive a mathematical model from the leading-edge force balance and the force-dependent polymerization rate. It reproduces quantitatively our measured adhesion–velocity relation and results with keratocytes, PtK1 cells, and CHO cells. Our results confirm that the force pushing the leading-edge membrane drives lamellipodial retrograde flow. Forces resisting motion originate along the whole cell length. All motion-related forces are controlled by adhesion and velocity, which allows motion, even with higher Fibronectin density at the rear than at the front. We find the pathway from Fibronectin density to adhesion structures to involve strong positive feedbacks. Suppressing myosin activity reduces the positive feedback. At transitions between different Fibronectin densities, steady motion is perturbed and leads to changes of cell length and front and rear velocity. Cells exhibit an intrinsic length set by adhesion strength, which, together with the length dynamics, suggests a spring-like front–rear interaction force. We provide a quantitative mechanistic picture of the adhesion–velocity relation and cell response to adhesion changes integrating force-dependent polymerization, retrograde flow, positive feedback from integrin to adhesion structures, and spring-like front–rear interaction. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell adhesion; Cell motility; Cellular biophysics; Surface micropattern
语种	英语
scopus关键词	fibronectin; integrin; myosin; animal cell; Article; biphasic adhesion velocity relation; cell adhesion; cell interaction; cell motility; CHO cell line; controlled study; cornea cell; human; human cell; lamellipodium; mathematical model; MDA-MB-231 cell line; mesenchyme cell; molecular dynamics; nonhuman; polymerization; positive feedback; priority journal
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180877
作者单位	Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, 80539, Germany; Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, 13125, Germany; Department of Physics, Humboldt University, Berlin, 12489, Germany
推荐引用方式 GB/T 7714	Schreiber C.,Amiri B.,Heyn J.C.J.,等. On the adhesion–velocity relation and length adaptation of motile cells on stepped fibronectin lanes[J],2021,118(4).
APA	Schreiber C.,Amiri B.,Heyn J.C.J.,Rädler J.O.,&Falcke M..(2021).On the adhesion–velocity relation and length adaptation of motile cells on stepped fibronectin lanes.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Schreiber C.,et al."On the adhesion–velocity relation and length adaptation of motile cells on stepped fibronectin lanes".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).