气候变化领域集成服务门户(CCPortal): Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM

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DOI	10.1073/pnas.2021180118
	Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM
	Zheng W.; Li F.; Ding Z.; Liu H.; Zhu L.; Xu C.; Li J.; Gao Q.; Wang Y.; Fu Z.; Peng C.; Yan X.; Zhu X.; Cong Y.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	The radial spoke (RS) heads of motile cilia and flagella contact projections of the central pair (CP) apparatus to coordinate motility, but the morphology is distinct for protozoa and metazoa. Here we show the murine RS head is compositionally distinct from that of Chlamydomonas. Our reconstituted murine RS head core complex consists of Rsph1, Rsph3b, Rsph4a, and Rsph9, lacking Rsph6a and Rsph10b, whose orthologs exist in the protozoan RS head. We resolve its cryo-electron microscopy (cryo-EM) structure at 3.2-Å resolution. Our atomic model further reveals a twofold symmetric brake pad-shaped structure, in which Rsph4a and Rsph9 form a compact body extended laterally with two long arms of twisted Rsph1 β-sheets and potentially connected dorsally via Rsph3b to the RS stalk. Furthermore, our modeling suggests that the core complex contacts the periodic CP projections either rigidly through its tooth-shaped Rsph4a regions or elastically through both arms for optimized RS–CP interactions and mechanosignal transduction. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cilia; Cryo-EM; Flagella; Primary ciliary dyskinesia; Radial spoke
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180852
作者单位	State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100049, China; State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, 200031, China; National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Shanghai, 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China; Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, 201210, China
推荐引用方式 GB/T 7714	Zheng W.,Li F.,Ding Z.,et al. Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM[J],2021,118(4).
APA	Zheng W..,Li F..,Ding Z..,Liu H..,Zhu L..,...&Cong Y..(2021).Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Zheng W.,et al."Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).