气候变化领域集成服务门户(CCPortal): Efficient detection and post-surgical monitoring of colon cancer with a multi-marker DNA methylation liquid biopsy

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DOI	10.1073/pnas.2017421118
	Efficient detection and post-surgical monitoring of colon cancer with a multi-marker DNA methylation liquid biopsy
	Jin S.; Zhu D.; Shao F.; Chen S.; Guo Y.; Li K.; Wang Y.; Ding R.; Gao L.; Ma W.; Lu T.; Li D.; Zhang Z.; Cai S.; Liang X.; Song H.; Ji L.; Li J.; Zheng Z.; Jiang F.; Wu X.; Luan J.; Zhang H.; Yang Z.; Cantor C.R.; Xu C.; Ding C.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	Multiplex assays, involving the simultaneous use of multiple circulating tumor DNA (ctDNA) markers, can improve the performance of liquid biopsies so that they are highly predictive of cancer recurrence. We have developed a single-tube methylation-specific quantitative PCR assay (mqMSP) that uses 10 different methylation markers and is capable of quantitative analysis of plasma samples with as little as 0.05% tumor DNA. In a cohort of 179 plasma samples from colorectal cancer (CRC) patients, adenoma patients, and healthy controls, the sensitivity and specificity of the mqMSP assay were 84.9% and 83.3%, respectively. In a head-to-head comparative study, the mqMSP assay also performed better for detecting early-stage (stage I and II) and premalignant polyps than a published SEPT9 assay. In an independent longitudinal cohort of 182 plasma samples (preoperative, postoperative, and follow-up) from 82 CRC patients, the mqMSP assay detected ctDNA in 73 (89.0%) of the preoperative plasma samples. Postoperative detection of ctDNA (within 2 wk of surgery) identified 11 of the 20 recurrence patients and was associated with poorer recurrence-free survival (hazard ratio, 4.20; P = 0.0005). With subsequent longitudinal monitoring, 14 patients (70%) had detectable ctDNA before recurrence, with a median lead time of 8.0 mo earlier than seen with radiologic imaging. The mqMSP assay is cost-effective and easily implementable for routine clinical monitoring of CRC recurrence, which can lead to better patient management after surgery. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Circulating tumor DNA; Colorectal cancer; DNA methylation; Liquid biopsy; Recurrence
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180829
作者单位	School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; Department of Colorectal Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Zhejiang Clinical Research Center of Minimally Invasive Diagnosis and Treatment of Abdominal Diseases, Zhejiang, 325000, China; Wenzhou Clinical Research Center of Colorectal Cancer, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; Department of Gastroenterology, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Central Laboratory, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States
推荐引用方式 GB/T 7714	Jin S.,Zhu D.,Shao F.,et al. Efficient detection and post-surgical monitoring of colon cancer with a multi-marker DNA methylation liquid biopsy[J],2021,118(5).
APA	Jin S..,Zhu D..,Shao F..,Chen S..,Guo Y..,...&Ding C..(2021).Efficient detection and post-surgical monitoring of colon cancer with a multi-marker DNA methylation liquid biopsy.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	Jin S.,et al."Efficient detection and post-surgical monitoring of colon cancer with a multi-marker DNA methylation liquid biopsy".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).