气候变化领域集成服务门户(CCPortal): Direct evidence that KNDy neurons maintain gonadotropin pulses and folliculogenesis as the GnRH pulse generator

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DOI	10.1073/pnas.2009156118
	Direct evidence that KNDy neurons maintain gonadotropin pulses and folliculogenesis as the GnRH pulse generator
	Nagae M.; Uenoyama Y.; Okamoto S.; Tsuchida H.; Ikegami K.; Goto T.; Majarune S.; Nakamura S.; Sanbo M.; Hirabayashi M.; Kobayashi K.; Inoue N.; Tsukamura H.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	The gonadotropin-releasing hormone (GnRH) pulse is fundamental for mammalian reproduction: GnRH pulse regimens are needed as therapies for infertile women as continuous GnRH treatment paradoxically inhibits gonadotropin release. Circumstantial evidence suggests that the hypothalamic arcuate KNDy neurons expressing kisspeptin (encoded by Kiss1), neurokinin B (encoded by Tac3), and dynorphin A serve as a GnRH pulse generator; however, no direct evidence is currently available. Here, we show that rescuing >20% KNDy neurons by transfecting Kiss1 inside arcuate Tac3 neurons, but not outside of these neurons, recovered folliculogenesis and luteinizing hormone (LH) pulses, an indicator of GnRH pulses, in female global Kiss1 knockout (KO) rats and that >90% conditional arcuate Kiss1 KO in newly generated Kiss1-floxed rats completely suppressed LH pulses. These results first provide direct evidence that KNDy neurons are the GnRH pulse generator, and at least 20% of KNDy neurons are sufficient to maintain folliculogenesis via generating GnRH/gonadotropin pulses. © 2020 Proceedings of the National Academy of Sciences of the United States of America. All rights reserved.
英文关键词	Conditional Kiss1 KO; Kisspeptin; KNDy rescue; LH pulses; Neurokinin B
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180822
作者单位	Laboratory of Animal Reproduction, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan; Section of Mammalian Transgenesis, Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8787, Japan; Laboratory of Animal Health, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, 794-8555, Japan; Section of Viral Vector Development, Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8585, Japan
推荐引用方式 GB/T 7714	Nagae M.,Uenoyama Y.,Okamoto S.,et al. Direct evidence that KNDy neurons maintain gonadotropin pulses and folliculogenesis as the GnRH pulse generator[J],2021,118(5).
APA	Nagae M..,Uenoyama Y..,Okamoto S..,Tsuchida H..,Ikegami K..,...&Tsukamura H..(2021).Direct evidence that KNDy neurons maintain gonadotropin pulses and folliculogenesis as the GnRH pulse generator.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	Nagae M.,et al."Direct evidence that KNDy neurons maintain gonadotropin pulses and folliculogenesis as the GnRH pulse generator".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).