气候变化领域集成服务门户(CCPortal): DNA methylation-linked chromatin accessibility affects genomic architecture in Arabidopsis

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DOI	10.1073/pnas.2023347118
	DNA methylation-linked chromatin accessibility affects genomic architecture in Arabidopsis
	Zhong Z.; Feng S.; Duttke S.H.; Potok M.E.; Zhang Y.; Gallego-Bartolomé J.; Liu W.; Jacobsen S.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	DNA methylation is a major epigenetic modification found across species and has a profound impact on many biological processes. However, its influence on chromatin accessibility and higher-order genome organization remains unclear, particularly in plants. Here, we present genome-wide chromatin accessibility profiles of 18 Arabidopsis mutants that are deficient in CG, CHG, or CHH DNA methylation. We find that DNA methylation in all three sequence contexts impacts chromatin accessibility in heterochromatin. Many chromatin regions maintain inaccessibility when DNA methylation is lost in only one or two sequence contexts, and signatures of accessibility are particularly affected when DNA methylation is reduced in all contexts, suggesting an interplay between different types of DNA methylation. In addition, we found that increased chromatin accessibility was not always accompanied by increased transcription, suggesting that DNA methylation can directly impact chromatin structure by other mechanisms. We also observed that an increase in chromatin accessibility was accompanied by enhanced long-range chromatin interactions. Together, these results provide a valuable resource for chromatin architecture and DNA methylation analyses and uncover a pivotal role for methylation in the maintenance of heterochromatin inaccessibility. © 2020 Proceedings of the National Academy of Sciences of the United States of America. All rights reserved.
英文关键词	Chromatin accessibility; DNA methylation; Epigenetics
语种	英语
scopus关键词	Arabidopsis; article; chromatin structure; DNA methylation; epigenetics; heterochromatin; nonhuman; transcription initiation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180805
作者单位	Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, United States; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, United States; Department of Medicine, University of California San Diego, San diego, CA 92093, United States; Department of Orthopedic, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310029, China; Zhejiang University, University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University, Haining, 314400, China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310029, China; Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, United States
推荐引用方式 GB/T 7714	Zhong Z.,Feng S.,Duttke S.H.,et al. DNA methylation-linked chromatin accessibility affects genomic architecture in Arabidopsis[J],2021,118(5).
APA	Zhong Z..,Feng S..,Duttke S.H..,Potok M.E..,Zhang Y..,...&Jacobsen S.E..(2021).DNA methylation-linked chromatin accessibility affects genomic architecture in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	Zhong Z.,et al."DNA methylation-linked chromatin accessibility affects genomic architecture in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).