气候变化领域集成服务门户(CCPortal): A protease-mediated mechanism regulates the cytochrome c6/plastocyanin switch in Synechocystis sp. PCC 6803

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DOI	10.1073/pnas.2017898118
	A protease-mediated mechanism regulates the cytochrome c6/plastocyanin switch in Synechocystis sp. PCC 6803
	García-Cañas R.; Giner-Lamia J.; Florencio F.J.; López-Maury L.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	After the Great Oxidation Event (GOE), iron availability was greatly decreased, and photosynthetic organisms evolved several alternative proteins and mechanisms. One of these proteins, plastocyanin, is a type I blue-copper protein that can replace cytochrome c6 as a soluble electron carrier between cytochrome b6f and photosystem I. In most cyanobacteria, expression of these two alternative proteins is regulated by copper availability, but the regulatory system remains unknown. Herein, we provide evidence that the regulatory system is composed of a BlaI/CopY-family transcription factor (PetR) and a BlaR-membrane protease (PetP). PetR represses petE (plastocyanin) expression and activates petJ (cytochrome c6), while PetP controls PetR levels in vivo. Using whole-cell extracts, we demonstrated that PetR degradation requires both PetP and copper. Transcriptomic analysis revealed that the PetRP system regulates only four genes (petE, petJ, slr0601, and slr0602), highlighting its specificity. Furthermore, the presence of petE and petRP in early branching cyanobacteria indicates that acquisition of these genes could represent an early adaptation to decreased iron bioavailability following the GOE. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cyanobacteria , cytochrome c6 , plastocyanin
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180804
作者单位	Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Sevilla, 41092, Spain; Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Biología, Universidad de Sevilla, Sevilla, 41012, Spain
推荐引用方式 GB/T 7714	García-Cañas R.,Giner-Lamia J.,Florencio F.J.,et al. A protease-mediated mechanism regulates the cytochrome c6/plastocyanin switch in Synechocystis sp. PCC 6803[J],2021,118(5).
APA	García-Cañas R.,Giner-Lamia J.,Florencio F.J.,&López-Maury L..(2021).A protease-mediated mechanism regulates the cytochrome c6/plastocyanin switch in Synechocystis sp. PCC 6803.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	García-Cañas R.,et al."A protease-mediated mechanism regulates the cytochrome c6/plastocyanin switch in Synechocystis sp. PCC 6803".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).