气候变化领域集成服务门户(CCPortal): Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid

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DOI	10.1073/pnas.2019622118
	Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid
	Mark Wainwright S.; Hopkins B.R.; Mendes C.C.; Sekar A.; Kroeger B.; Hellberg J.E.E.U.; Fan S.-J.; Pavey A.; Marie P.P.; Leiblich A.; Sepil I.; Charles P.D.; Thézénas M.L.; Fischer R.; Kessler B.M.; Gandy C.; Corrigan L.; Patel R.; Wigby S.; Morris J.F.; Goberdhan D.C.I.; Wilson C.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	Seminal fluid plays an essential role in promoting male reproductive success and modulating female physiology and behavior. In the fruit fly, Drosophila melanogaster, Sex Peptide (SP) is the best-characterized protein mediator of these effects. It is secreted from the paired male accessory glands (AGs), which, like the mammalian prostate and seminal vesicles, generate most of the seminal fluid contents. After mating, SP binds to spermatozoa and is retained in the female sperm storage organs. It is gradually released by proteolytic cleavage and induces several long-term postmating responses, including increased ovulation, elevated feeding, and reduced receptivity to remating, primarily signaling through the SP receptor (SPR). Here, we demonstrate a previously unsuspected SPR-independent function for SP. We show that, in the AG lumen, SP and secreted proteins with membrane-binding anchors are carried on abundant, large neutral lipid-containing microcarriers, also found in other SP-expressing Drosophila species. These microcarriers are transferred to females during mating where they rapidly disassemble. Remarkably, SP is a key microcarrier assembly and disassembly factor. Its absence leads to major changes in the seminal proteome transferred to females upon mating. Males expressing nonfunctional SP mutant proteins that affect SP’s binding to and release from sperm in females also do not produce normal microcarriers, suggesting that this male-specific defect contributes to the resulting widespread abnormalities in ejaculate function. Our data therefore reveal a role for SP in formation of seminal macromolecular assemblies, which may explain the presence of SP in Drosophila species that lack the signaling functions seen in D. melanogaster. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Reproduction; Secretion; Seminal proteins; Sex peptide; Triacylglycerides
语种	英语
scopus关键词	animal cell; animal experiment; animal tissue; Article; controlled study; Drosophila melanogaster; female; gender; macromolecule; male; microcarrier culture; mutant; nonhuman; organ preservation; priority journal; protein binding; protein function; protein metabolism; seminal plasma; seminal vesicle; signal transduction; sperm preservation; spermatozoon
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180785
作者单位	Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3QX, United Kingdom; Department of Zoology, University of Oxford, Oxford, OX1 3PS, United Kingdom; Department of Evolution and Ecology, University of California, Davis, CA 95616, United States; Target Discovery Institute Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, United Kingdom; Applied Zoology, Faculty of Biology, Technische Universität Dresden, Dresden, D-01069, Germany; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, L69 7ZB, United Kingdom
推荐引用方式 GB/T 7714	Mark Wainwright S.,Hopkins B.R.,Mendes C.C.,et al. Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid[J],2021,118(5).
APA	Mark Wainwright S..,Hopkins B.R..,Mendes C.C..,Sekar A..,Kroeger B..,...&Wilson C..(2021).Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	Mark Wainwright S.,et al."Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).