气候变化领域集成服务门户(CCPortal): Comparative analysis of embryo proper and suspensor transcriptomes in plant embryos with different morphologies

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DOI	10.1073/pnas.2024704118
	Comparative analysis of embryo proper and suspensor transcriptomes in plant embryos with different morphologies
	Chen M.; Lin J.-Y.; Wu X.; Apuya N.R.; Henry K.F.; Le B.H.; Bui A.Q.; Pelletier J.M.; Cokus S.; Pellegrini M.; Harada J.J.; Goldberg R.B.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	An important question is what genes govern the differentiation of plant embryos into suspensor and embryo proper regions following fertilization and division of the zygote. We compared embryo proper and suspensor transcriptomes of four plants that vary in embryo morphology within the suspensor region. We determined that genes encoding enzymes in several metabolic pathways leading to the formation of hormones, such as gibberellic acid, and other metabolites are up-regulated in giant scarlet runner bean and common bean suspensors. Genes involved in transport and Golgi body organization are up-regulated within the suspensors of these plants as well, strengthening the view that giant specialized suspensors serve as a hormone factory and a conduit for transferring substances to the developing embryo proper. By contrast, genes controlling transcriptional regulation, development, and cell division are up-regulated primarily within the embryo proper. Transcriptomes from less specialized soybean and Arabidopsis suspensors demonstrated that fewer genes encoding metabolic enzymes and hormones are up-regulated. Genes active in the embryo proper, however, are functionally similar to those active in scarlet runner bean and common bean embryo proper regions. We uncovered a set of suspensor- and embryo proper-specific transcription factors (TFs) that are shared by all embryos irrespective of morphology, suggesting that they are involved in early differentiation processes common to all plants. Chromatin immunoprecipitation sequencing (ChIP-Seq) experiments with scarlet runner bean and soybean WOX9, an up-regulated suspensor TF, gained entry into a regulatory network important for suspensor development irrespective of morphology. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	ChIP-Seq; Embryo proper; Laser capture microdissection; Suspensor; Transcriptome
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180765
作者单位	Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, United States; Department of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, United States; Academia Sinica, Biotechnology Center in Southern Taiwan, Tainan, 74145, Taiwan; Institute of Citrus Science, Huazhong Agricultural University, Wuhan, 430070, China; Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, United States; Inivata Ltd., Research Triangle ParkNC 27560, United States
推荐引用方式 GB/T 7714	Chen M.,Lin J.-Y.,Wu X.,et al. Comparative analysis of embryo proper and suspensor transcriptomes in plant embryos with different morphologies[J],2021,118(6).
APA	Chen M..,Lin J.-Y..,Wu X..,Apuya N.R..,Henry K.F..,...&Goldberg R.B..(2021).Comparative analysis of embryo proper and suspensor transcriptomes in plant embryos with different morphologies.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Chen M.,et al."Comparative analysis of embryo proper and suspensor transcriptomes in plant embryos with different morphologies".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).