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DOI	10.1073/pnas.2019628118
	A stable antimicrobial peptide with dual functions of treating and preventing citrus Huanglongbing
	Huang C.-Y.; Araujo K.; Sánchez J.N.; Kund G.; Trumble J.; Roper C.; Godfrey K.E.; Jin H.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Citrus Huanglongbing (HLB), caused by a vector-transmitted phloem-limited bacterium Candidatus Liberibacter asiaticus (CLas), is the most devastating citrus disease worldwide. Currently, there are no effective strategies to prevent infection or to cure HLB-positive trees. Here, using comparative analysis between HLB-sensitive citrus cultivars and HLB-tolerant citrus hybrids and relatives, we identified a novel class of stable antimicrobial peptides (SAMPs). The SAMP from Microcitrus australiasica can rapidly kill Liberibacter crescens (Lcr), a culturable Liberibacter strain, and inhibit infections of CLas and CL. solanacearum in plants. In controlled greenhouse trials, SAMP not only effectively reduced CLas titer and disease symptoms in HLB-positive trees but also induced innate immunity to prevent and inhibit infections. Importantly, unlike antibiotics, SAMP is heat stable, making it better suited for field applications. Spray-applied SAMP was taken up by citrus leaves, stayed stable inside the plants for at least a week, and moved systemically through the vascular system where CLas is located. We further demonstrate that SAMP is most effective on α-proteobacteria and causes rapid cytosol leakage and cell lysis. The α-helix-2 domain of SAMP is sufficient to kill Lcr. Future field trials will help determine the efficacy of SAMP in controlling HLB and the ideal mode of application. © This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Antimicrobial peptide; Candidatus liberibacter; Citrus huanglongbing; Microcitrus; Plant immunity
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180762
作者单位	Department of Microbiology and Plant Pathology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, United States; Contained Research Facility, University of California, Davis, CA 95616, United States; Department of Entomology, University of California, Riverside, CA 92521, United States
推荐引用方式 GB/T 7714	Huang C.-Y.,Araujo K.,Sánchez J.N.,et al. A stable antimicrobial peptide with dual functions of treating and preventing citrus Huanglongbing[J],2021,118(6).
APA	Huang C.-Y..,Araujo K..,Sánchez J.N..,Kund G..,Trumble J..,...&Jin H..(2021).A stable antimicrobial peptide with dual functions of treating and preventing citrus Huanglongbing.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Huang C.-Y.,et al."A stable antimicrobial peptide with dual functions of treating and preventing citrus Huanglongbing".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).